State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Department of Respiratory Medicine, Center for Pathogen Biology and Infectious Diseases, Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital of Jilin University, Changchun, China.
Front Immunol. 2023 Jun 28;14:1209879. doi: 10.3389/fimmu.2023.1209879. eCollection 2023.
, one species of spp., has the greatest impact on human health and is the main cause of bacterial sexually transmitted diseases and preventable blindness among all spp. species. The obligate intracellular parasitism and unique biphasic developmental cycle of are the main barriers for the development of tools of genetic manipulation. The past decade has witnessed significant gains in genetic manipulation of , including chemical mutagenesis, group II intron-based targeted gene knockout, fluorescence-reported allelic exchange mutagenesis (FRAEM), CRISPR interference (CRISPRi) and the recently developed transposon mutagenesis. In this review, we discuss the current status of genetic manipulations of and highlights new challenges in the nascent field of genetics.
，属于 spp. 的一个种，对人类健康的影响最大，是所有 spp. 种中导致细菌性性传播疾病和可预防失明的主要原因。的专性细胞内寄生和独特的两相发育周期是开发遗传操作工具的主要障碍。在过去的十年中，在 的遗传操作方面取得了重大进展，包括化学诱变、基于第二类内含子的靶向基因敲除、荧光报告等位基因交换诱变（FRAEM）、CRISPR 干扰（CRISPRi）和最近开发的转座子诱变。在这篇综述中，我们讨论了 的遗传操作的现状，并强调了这一新兴领域的新挑战。
