Yoshida H, Miyamoto K, Yoshida T, Ogawa H, Imamura K
J Androl. 1986 Jul-Aug;7(4):220-3. doi: 10.1002/j.1939-4640.1986.tb00917.x.
A cup-shaped silicon prosthesis was developed to treat patients with excretory azoospermia and chemical management of the aspirated spermatozoa was attempted prior to cervical insemination of the wives. These cup-shaped prostheses were implanted eight times in six patients, four of whom had congenital absence and two of whom had bilateral inflammatory obstruction of the vas deferens. The surgical technique was based on the method of Cruz (1980), which involved wrapping the tunica vaginalis over the top of the prosthesis. Aspirated spermatozoa were incubated in a modified Ringer's solution at 37 C for 30 minutes and used for the cervical insemination of the wives. Epididymal spermatozoa could be collected from all patients 10 to 20 weeks after the operation. The percentage of motile aspirated spermatozoa ranged from 0 to 20%. In two cases, the percentage of motile spermatozoa aspirated from the prosthesis increased markedly from 5 and 10% to 55 and 60%, respectively, after incubation of the spermatozoa in the medium. Cervical inseminations were tried in all of the wives from two to four times, but no pregnancy resulted during this study.
开发了一种杯状硅假体来治疗排泄性无精子症患者，并在对其妻子进行宫颈授精之前尝试对吸出的精子进行化学处理。这些杯状假体在6名患者中植入了8次，其中4名患者先天性输精管缺如，2名患者双侧输精管炎性梗阻。手术技术基于克鲁兹（1980年）的方法，即把鞘膜包裹在假体顶部。吸出的精子在改良的林格氏液中于37℃孵育30分钟，然后用于对其妻子进行宫颈授精。术后10至20周，所有患者均能采集到附睾精子。吸出的活动精子百分比在0%至20%之间。在两例患者中，精子在培养基中孵育后，从假体吸出的活动精子百分比分别从5%和10%显著增加到55%和60%。对所有患者的妻子都进行了两到四次宫颈授精尝试，但在本研究期间均未受孕。
