Genesis, Beijing, China.
Methods Mol Biol. 2023;2695:9-25. doi: 10.1007/978-1-0716-3346-5_2.
Liquid biopsy has obvious advantages over invasive sampling methods. Cell-free DNA is now widely used in prenatal and cancer diagnosis. Cell-free RNA is relatively unstable but could contain potential biomarkers, and some studies have demonstrated that cfRNA sequencing may be developed to be a promising diagnostic assay for disease detection. In this chapter, we introduce a modified protocol for the detection of cfRNA based on next-generation sequencing (NGS). This protocol has been specifically optimized for low-input, fragmented cfRNA samples.
液体活检相对于有创采样方法具有明显优势。游离 DNA 目前已广泛应用于产前诊断和癌症诊断。游离 RNA 相对不稳定，但可能包含潜在的生物标志物，一些研究表明，cfRNA 测序可能发展成为一种用于疾病检测的很有前途的诊断检测方法。在本章中，我们介绍了一种基于下一代测序（NGS）的 cfRNA 检测改良方案。该方案专门针对低输入、碎片化的 cfRNA 样本进行了优化。
