Lung Biology Center, University of California, San Francisco, UCSF Box 3118, San Francisco, CA 94143, USA.
Division of Pulmonary, Critical Care, Allergy and Sleep Medicine, Department of Medicine, University of California, San Francisco, UCSF Box 0130, San Francisco, CA 94143, USA.
Cell Rep. 2018 Oct 30;25(5):1346-1358. doi: 10.1016/j.celrep.2018.10.014.
Extracellular microRNAs (miRNAs) and other small RNAs are implicated in cellular communication and may be useful as disease biomarkers. We systematically compared small RNAs in 12 human biofluid types using RNA sequencing (RNA-seq). miRNAs and tRNA-derived RNAs (tDRs) accounted for the majority of mapped reads in all biofluids, but the ratio of miRNA to tDR reads varied from 72 in plasma to 0.004 in bile. miRNA levels were highly correlated across all biofluids, but levels of some miRNAs differed markedly between biofluids. tDR populations differed extensively between biofluids. Y RNA fragments were seen in all biofluids and accounted for >10% of reads in blood plasma, serum, and cerebrospinal fluid (CSF). Reads mapping exclusively to Piwi-interacting RNAs (piRNAs) were very rare, except in seminal plasma. These results demonstrate extensive differences in small RNAs between human biofluids and provide a useful resource for investigating extracellular RNA biology and developing biomarkers.
细胞外 microRNAs (miRNAs) 和其他小 RNA 参与细胞通讯，可能可用作疾病生物标志物。我们使用 RNA 测序 (RNA-seq) 系统地比较了 12 种人体生物流体中的小 RNA。miRNAs 和 tRNA 衍生的 RNA (tDR) 在所有生物流体中占映射读数的大部分，但 miRNA 与 tDR 读数的比例从血浆中的 72 变化到胆汁中的 0.004。miRNA 水平在所有生物流体中高度相关，但一些 miRNA 的水平在生物流体之间差异显著。tDR 群体在生物流体之间差异很大。Y RNA 片段存在于所有生物流体中，占血液、血清和脑脊液 (CSF) 中读取的 >10%。专门映射到 Piwi 相互作用 RNA (piRNAs) 的读取非常罕见，除了在精液中。这些结果表明人类生物流体之间的小 RNA 存在广泛差异，并为研究细胞外 RNA 生物学和开发生物标志物提供了有用的资源。
