使用谷胱甘肽 S-转移酶（GST）下拉实验技术检测蛋白质-蛋白质相互作用。

Detection of Protein-Protein Interactions Using Glutathione-S-Transferase (GST) Pull-Down Assay Technique.

机构信息

Department of Plant Pathology, Washington State University, Pullman, WA, USA.

出版信息

Methods Mol Biol. 2023;2690:111-115. doi: 10.1007/978-1-0716-3327-4_10.

Abstract

Pull-down assay is a technique to analyze direct protein-protein interaction under in vitro condition. Also, this technique is appropriate for investigating the direct interaction between two purified proteins. Glutathione-s-transferase (GST) protein is a widely used affinity tag for affinity purification. In this chapter, we explain the widely used GST pull-down assay to identify the protein-protein interaction between purified proteins.

摘要

下拉实验是一种在体外条件下分析直接蛋白质-蛋白质相互作用的技术。此外，该技术也适用于研究两种纯化蛋白之间的直接相互作用。谷胱甘肽 S-转移酶 (GST) 蛋白是一种广泛用于亲和纯化的亲和标签。在本章中，我们将解释广泛使用的 GST 下拉实验，以鉴定纯化蛋白之间的蛋白质-蛋白质相互作用。