Center for Experimental Medicine, Institute for Clinical and Experimental Medicine - IKEM, Prague, Czech Republic.
Department of Pathophysiology, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic.
J Physiol Pharmacol. 2023 Apr;74(2). doi: 10.26402/jpp.2023.2.02. Epub 2023 Jul 10.
The pressure-volume (PV) analysis is used for an accurate assessment of load-independent cardiac function and is important for the study of cardiovascular diseases and various therapeutic modalities. PV analysis is often performed on one of the ventricles, or on both ventricles but sequentially. Since both ventricles interact with each other and their functions are mutually interdependent, especially in various disease conditions such as pulmonary hypertension or heart failure, it is important to quantify the function of both ventricles at the same time. Therefore, our aim was to describe a standardized protocol for simultaneous right (RV) and left (LV) ventricle of PV analysis, including an especially controllable preload reduction manoeuver. Our second aim was to test whether simultaneous catheterization of both LV and RV is necessary for the determination of biventricular PV relationship compared to sequential measurement of both ventricles separately. In this article, we showed the feasibility and the value of simultaneous biventricular PV analysis in the measurement of contractility parameters (end-systolic pressure-volume relationship (ESPVR), ventricular pressure over time (dP/dt)max, divided by end-diastolic volume (dP/dt max-EDV)) with a comparison to the sequential measurement of the RV and LV ventricles separately. We described in detail the protocol for simultaneous biventricular PV analysis in rats using a pair of conductance-micromanometer catheters with a preload-reducing manoeuver using balloon catheter inflation in the inferior vena cava. We also described technical tips and show examples of PV loop data obtained in normotensive and hypertensive rats, with and without heart failure due to volume overload. This protocol could be useful for scientists studying hemodynamics and cardiac contractility in various models of cardiovascular diseases with a focus on biventricular differences and ventricular interdependence.
压力-容积（PV）分析用于准确评估负荷独立的心脏功能，对于心血管疾病和各种治疗方式的研究非常重要。PV 分析通常在一个心室或两个心室上进行，但顺序不同。由于两个心室相互作用，它们的功能相互依存，特别是在各种疾病情况下，如肺动脉高压或心力衰竭，同时量化两个心室的功能非常重要。因此，我们的目的是描述一种标准化的同时右（RV）和左（LV）心室 PV 分析协议，包括一种特别可控的前负荷降低操作。我们的第二个目的是测试与分别顺序测量两个心室相比，同时导管插入两个 LV 和 RV 是否有必要确定双心室 PV 关系。在本文中，我们展示了同时双心室 PV 分析在测量收缩性参数（收缩末期压力-容积关系（ESPVR）、心室压力随时间变化（dP/dt）max，除以舒张末期容积（dP/dt max-EDV））方面的可行性和价值，并与单独顺序测量 RV 和 LV 心室进行了比较。我们详细描述了使用一对带有前负荷降低操作的电导-微压力计导管在大鼠中同时进行双心室 PV 分析的协议，该操作使用下腔静脉气球导管充气来实现。我们还描述了技术要点，并展示了在正常血压和高血压大鼠中以及由于容量超负荷导致心力衰竭时获得的 PV 环数据的示例。该协议可用于研究各种心血管疾病模型中血液动力学和心脏收缩性的科学家，重点关注双心室差异和心室相互依存。
