Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
J Physiol Pharmacol. 2023 Apr;74(2). doi: 10.26402/jpp.2023.2.09. Epub 2023 Jul 10.
The optimal environment in the oviduct is created by adjusting its ultrastructure and secretory activity to serve the most suitable protection of gametes and to support embryo development. Through gametes/embryo's presence inside the oviduct, the oviductal transcriptomic profile may be altered, and these changes may be caused by DNA methylation. The results of the present study documented that in the epithelial cells of the ampulla and isthmus of the oviducts collected from pigs during the peri-conceptional period, the most differentially expressed genes (DEGs) were down-regulated. Identified DEGs were classified into gene ontology categories as well as annotated into different biological pathways. From evaluated DEGs, genes important for embryo development were selected and the level of DNA methylation was determined. It was documented CLDN18, MUC1, CYP19A3, SOCS1, and ESR1 methylation level have been altered. The presence of embryos in the oviduct changed the transcriptomic profile and the level of DNA methylation in the epithelial cells of ampulla and isthmus during the peri-conceptional period.
输卵管中的最佳环境是通过调整其超微结构和分泌活动来创造的，以提供对配子的最适宜保护，并支持胚胎发育。通过配子/胚胎在输卵管内的存在，输卵管转录组谱可能会发生改变，而这些变化可能是由 DNA 甲基化引起的。本研究的结果记录了在受孕前阶段从猪输卵管壶腹部和峡部采集的上皮细胞中，差异表达基因（DEGs）被下调。鉴定出的 DEGs 被分为基因本体论类别，并注释到不同的生物学途径中。从评估的 DEGs 中，选择了对胚胎发育重要的基因，并确定了 DNA 甲基化的水平。有文献记录 CLDN18、MUC1、CYP19A3、SOCS1 和 ESR1 的甲基化水平发生了改变。胚胎在输卵管内的存在改变了受孕前阶段输卵管壶腹部和峡部上皮细胞的转录组谱和 DNA 甲基化水平。
