School of Materials Science and Engineering, Shanghai University of Engineering Science, Shanghai, 201620, China.
School of Materials Science and Engineering, Shanghai University of Engineering Science, Shanghai, 201620, China.
Anal Chim Acta. 2023 Sep 15;1274:341584. doi: 10.1016/j.aca.2023.341584. Epub 2023 Jul 3.
The level of uric acid is crucial to human health. Octahedral oxygen vacancy MnCoO/Ag (V-MnCoO/Ag) nanozyme was successfully prepared by simple hydrothermal, calcination and self-reduction methods. V-MnCoO/Ag nanozyme is rich in Mn/Mn and C/C redox electron pairs, large specific surface area and oxygen vacancies. V-MnCoO/Ag nanozyme showed high uricase-like activity and peroxidase-like activity. At the same time, the SERS signal of the detected molecule could be significantly enhanced after the catalytic reaction of the V-MnCoO/Ag nanozyme. The K values of V-MnCoO/Ag nanozyme for HO and TMB were 0.04 mM and 0.027 mM respectively. Based on the uric acid oxidase-like and peroxidase-like activities of V-MnCoO/Ag, we developed a label-free, sensitive, and reliable SERS uric acid detection system. The detection linear range of uric acid is 0.01 μM-1000 μM and the detection of limit is 7.8 × 10 M. The results show that the sensing system has good accuracy, sensitivity, selectivity, and stability. It can be applied to the determination of samples under different conditions. This study provides profound insights into the design of enzyme-like activity regulation and SERS properties regulation of nanozymes, provides guidance for the study of reaction kinetics and catalytic mechanism of nanozymes, and has broad application prospects in the field of nanozymes and SERS sensing analysis.
尿酸水平对人类健康至关重要。通过简单的水热、煅烧和自还原方法成功制备了八面体氧空位 MnCoO/Ag（V-MnCoO/Ag）纳米酶。V-MnCoO/Ag 纳米酶富含 Mn/Mn 和 C/C 氧化还原电子对、大比表面积和氧空位。V-MnCoO/Ag 纳米酶表现出高尿酸酶样活性和过氧化物酶样活性。同时，V-MnCoO/Ag 纳米酶催化反应后，检测分子的 SERS 信号可以得到显著增强。V-MnCoO/Ag 纳米酶对 HO 和 TMB 的 K 值分别为 0.04 mM 和 0.027 mM。基于 V-MnCoO/Ag 的尿酸氧化酶样和过氧化物酶样活性，我们开发了一种无标记、灵敏、可靠的 SERS 尿酸检测系统。尿酸的检测线性范围为 0.01 μM-1000 μM，检测限为 7.8×10^-7 M。结果表明，该传感系统具有良好的准确性、灵敏度、选择性和稳定性。它可以应用于不同条件下的样品测定。本研究为纳米酶酶样活性调节和 SERS 性质调节的设计提供了深入的见解，为纳米酶反应动力学和催化机制的研究提供了指导，并在纳米酶和 SERS 传感分析领域具有广阔的应用前景。
