Lavine M E, Clark R M, Hundrieser K E, Ferris A M
J Dairy Sci. 1986 Jul;69(7):1784-6. doi: 10.3168/jds.S0022-0302(86)80601-4.
Milk was collected from six mothers during their 8th wk of lactation to determine the diurnal variation of bile salt-stimulated lipase and serum-stimulated lipase. On the day of collection, one breast was completely emptied at 0600, 1000, 1400, 1800, and 2000 h. Activities of bile-stimulated lipase and serum-stimulated lipase in the milk were determined. Activity of bile-stimulated lipase was constant throughout the day, whereas serum-stimulated lipase activity varied significantly with time. Both activities differed significantly in milk from different women. Time of collection must be considered when measuring activity of serum-stimulated lipase but is not a major variable when measuring activity of bile-stimulated lipase in human milk. These results may be helpful when designing future studies involving these enzymes.
在六位母亲哺乳期的第8周收集乳汁，以确定胆汁盐刺激脂肪酶和血清刺激脂肪酶的日变化。在收集当天，分别于06:00、10:00、14:00、18:00和20:00将一侧乳房完全排空，并测定乳汁中胆汁刺激脂肪酶和血清刺激脂肪酶的活性。胆汁刺激脂肪酶的活性在一天中保持恒定，而血清刺激脂肪酶的活性随时间有显著变化。不同女性乳汁中的这两种酶活性差异显著。在测量血清刺激脂肪酶活性时必须考虑收集时间，但在测量人乳中胆汁刺激脂肪酶活性时，收集时间不是主要变量。这些结果可能有助于设计未来涉及这些酶的研究。
