College of Biology and Food Engineering, Changshu Institute of Technology, Changshu, P. R. China.
College of Food and Drug, Shanghai Zhongqiao Vocational and Technical University, Shanghai, P. R. China.
J Food Sci. 2023 Aug;88(8):3323-3331. doi: 10.1111/1750-3841.16681. Epub 2023 Jul 17.
In this study, "Xiahui 6" peaches were treated with 10 µL/L 1-methylcyclopropene (1-MCP) for 12 h and then stored at 20°C for 9 days; the regulation of 1-MCP on organic acids during storage was investigated through transcriptomic and metabolite analyses. Results showed that 1-MCP maintained higher gene expression of malate synthesis (PpPEPC1, PpPEPC2, and PpNAD-cytMDH) at the end of storage but extremely inhibited the gene expression of malate degradation (PpNADP-cytME) during storage, resulting that malate content in treated peaches was twice that of control group at day 7. Besides, the increasement of citrate synthesis and degradation-related genes (PpmitCS, PpcytACO, PpNAD-mitIDH, and PpNADP-cytIDH) at days 3 and 5 was postponed by 1-MCP treatment, accompanied by 0.5 times higher citrate content at day 7. Our results suggested that 1-MCP has inhibitory effects on both the synthesis and degradation of organic acids; however, the inhibitory effect of 1-MCP on organic acid degradation may be greater than that on organic acid synthesis. Practical Application: This study provides a theoretical basis for the application of 1-methylcyclopropene (1-MCP) in fruit preservation.
在这项研究中，对“霞辉 6 号”桃进行了 10μL/L 1-甲基环丙烯（1-MCP）处理 12 小时，然后在 20°C 下储存 9 天；通过转录组和代谢物分析研究了 1-MCP 对储存期间有机酸的调节作用。结果表明，1-MCP 在储存结束时维持较高的苹果酸合成基因表达（PpPEPC1、PpPEPC2 和 PpNAD-cytMDH），但在储存过程中极度抑制苹果酸降解基因表达（PpNADP-cytME），导致处理过的桃子中的苹果酸含量在第 7 天是对照组的两倍。此外，柠檬酸合成和降解相关基因（PpmitCS、PpcytACO、PpNAD-mitIDH 和 PpNADP-cytIDH）在第 3 天和第 5 天的增加被 1-MCP 处理推迟，第 7 天的柠檬酸含量增加了 0.5 倍。我们的结果表明，1-MCP 对有机酸的合成和降解均有抑制作用；然而，1-MCP 对有机酸降解的抑制作用可能大于对有机酸合成的抑制作用。实际应用：本研究为 1-甲基环丙烯（1-MCP）在水果保鲜中的应用提供了理论依据。
