Fleig W E, Lehmann H, Wagner H, Hoss G, Ditschuneit H
J Hepatol. 1986;3(1):19-26. doi: 10.1016/s0168-8278(86)80141-6.
The occurrence and activity of hepatic regenerative stimulator substance was investigated in the partially hepatectomized rabbit and related to biochemical and morphological parameters of liver regeneration. Male rabbits were 60% hepatectomized by excising the Spigelian, left lateral and left central lobes of the liver leaving the gallbladder in situ. [3H]Thymidine incorporation into DNA, the fraction of labelled hepatocyte nuclei, the fraction of mitoses and thymidine kinase activity rose from basal levels at 30-40 h after hepatectomy and increased up to 12-fold at 40-60 h. After 7 days, proliferation parameters returned to near prae-hepatectomy values and 82% of the initial liver mass was restored. Hepatic regenerative stimulator substance was biologically active when prepared from rabbit livers between 18-30 h after partial hepatectomy. At 12 and 30 h after intraperitoneal injection of the extract into normal rats, hepatic DNA synthesis was stimulated up to 2-fold in a dose-dependent fashion. The biological activity was protease-sensitive and thus depended on a protein component of the extract. The data demonstrate the existence of hepatic regenerative stimulator substance in regenerating rabbit liver and suggest that it is implicated in the regulation of liver growth after partial hepatectomy.
在部分肝切除的兔子中研究了肝再生刺激物质的产生和活性，并将其与肝再生的生化和形态学参数相关联。雄性兔子通过切除肝的斯皮格尔叶、左外侧叶和左中叶进行60%肝切除，保留胆囊原位。肝切除后30 - 40小时，[3H]胸苷掺入DNA、标记的肝细胞核分数、有丝分裂分数和胸苷激酶活性从基础水平上升，在40 - 60小时增加高达12倍。7天后，增殖参数恢复到接近肝切除前的值，初始肝脏质量的82%得以恢复。当从部分肝切除后18 - 30小时的兔肝脏中制备肝再生刺激物质时具有生物活性。在向正常大鼠腹腔注射提取物后12小时和30小时，肝DNA合成以剂量依赖方式被刺激高达2倍。其生物活性对蛋白酶敏感，因此依赖于提取物的蛋白质成分。这些数据证明在再生的兔肝脏中存在肝再生刺激物质，并表明它参与了部分肝切除后肝脏生长的调节。
