Moore D H
J Histochem Cytochem. 1979 Jan;27(1):305-10. doi: 10.1177/27.1.374592.
A new method for decomposing flow cytometry histograms of isolated human metaphase chromosomes is described and tested. The method is based on fitting a template, composed of the means of all chromosomes of a normal karyotype to the flow histogram. The utility of the method is demonstrated by application to flow measurements of chromosomes from a normal person and comparing the results with those obtained by conventional cytophotometry. The power of the method for detecting gross chromosomal abnormalities, such as trisomy 21, as well as more subtle variations such as a single translocation, is determined for simulated data.
本文描述并测试了一种用于分解分离的人类中期染色体流式细胞术直方图的新方法。该方法基于将由正常核型的所有染色体均值组成的模板拟合到流式直方图上。通过将该方法应用于正常人染色体的流式测量，并将结果与传统细胞光度法获得的结果进行比较，证明了该方法的实用性。对于模拟数据，确定了该方法检测21三体等明显染色体异常以及单个易位等更细微变异的能力。
