同时进行骨髓细胞的流式细胞术DNA和体积测量，作为大鼠白血病异常增殖模式的敏感指标。

Simultaneous flow cytometric DNA and volume measurements of bone marrow cells as sensitive indicator of abnormal proliferation patterns in rat leukemias.

作者信息

Valet G, Fischer B, Sundergeld A, Hanser G, Kachel V, Ruhenstroth-Bauer G

出版信息

J Histochem Cytochem. 1979 Jan;27(1):398-403. doi: 10.1177/27.1.374601.

DOI:10.1177/27.1.374601

Abstract

Simultaneous flow cytometric DNA and volume analysis of normal rat bone marrow cells shows three populations of nucleated cells with different mean volume. Each of these populations proliferates in a distinct cell cycle (alpha, beta, gamma). Normally the alpha-cell cycle has the highest amplitude, the beta-cell cycle is intermediate, and the gamma-cell cycle is low. The alpha-cell cycle was very significantly depressed and the beta + gamma-cell cycle was increased in three different rat leukemias (L5222, Shay, BNML), growing on three different rat strains (BDIX, Holtzmann, Brown Norway). The two parameter analysis further revealed that cells of the beta + gamma-cell cycle were slightly hyperdiploid and hypertetraploid in leukemic animals. The decrease of the alpha-cell cycle and the hyperploidies were more sensitive indicators for the abnormal proliferation pattern than the analysis of one parameter DNA distributions which remained within normal limits in all three leukemias.

摘要

对正常大鼠骨髓细胞进行流式细胞术同步DNA和体积分析，结果显示有三种具有不同平均体积的有核细胞群。这些细胞群中的每一个都在不同的细胞周期（α、β、γ）中增殖。正常情况下，α细胞周期的幅度最高，β细胞周期居中，γ细胞周期较低。在三种不同大鼠品系（BDIX、Holtzmann、Brown Norway）上生长的三种不同大鼠白血病（L5222、Shay、BNML）中，α细胞周期显著降低，β+γ细胞周期增加。双参数分析进一步显示，在白血病动物中，β+γ细胞周期的细胞略为超二倍体和超四倍体。与单参数DNA分布分析相比，α细胞周期的减少和超倍体是异常增殖模式更敏感的指标，在所有三种白血病中，单参数DNA分布均保持在正常范围内。