Armstrong D G
J Endocrinol. 1986 Aug;110(2):211-6. doi: 10.1677/joe.0.1100211.
Fowl ovarian ornithine decarboxylase activity was measured at 20, 10 and 3 h before an expected ovulation in granulosa and thecal tissues from follicles at various stages of development. An increase in the enzyme activity between 10 and 3 h before an expected ovulation was assumed to be caused by preovulatory increase in plasma LH concentration. The activity in granulosa tissue increased with increasing size of the follicle. In the largest (F1) follicle there was an 11-fold increase in granulosa ornithine decarboxylase specific activity between 10 and 3 h before ovulation. In the third (F3) and fifth (F5) largest follicles there was a 1.9- and 2-fold increase respectively. The enzyme activity in thecal tissue from the follicular hierarchy decreased with increasing size of the follicle and the F3 thecal preparation was the only tissue to respond to the preovulatory LH surge. In contrast, ornithine decarboxylase activity in thecal tissue from small (less than 5 mm) non-atretic follicles increased by two- to threefold after the preovulatory LH surge. The activity in atretic follicles of the same size was low and remained unchanged throughout the ovulatory cycle.
在预期排卵前20小时、10小时和3小时，测定了处于不同发育阶段卵泡的颗粒层和卵泡膜组织中家禽卵巢鸟氨酸脱羧酶的活性。假定在预期排卵前10小时至3小时之间酶活性的增加是由血浆促黄体生成素（LH）浓度的排卵前升高引起的。颗粒层组织中的活性随着卵泡大小的增加而增加。在最大的（F1）卵泡中，排卵前10小时至3小时之间颗粒层鸟氨酸脱羧酶的比活性增加了11倍。在第三大（F3）和第五大（F5）卵泡中，分别增加了1.9倍和2倍。卵泡等级结构中卵泡膜组织的酶活性随着卵泡大小的增加而降低，并且F3卵泡膜制剂是唯一对排卵前LH激增有反应的组织。相比之下，小（小于5毫米）的非闭锁卵泡的卵泡膜组织中的鸟氨酸脱羧酶活性在排卵前LH激增后增加了两到三倍。相同大小的闭锁卵泡中的活性较低，并且在整个排卵周期中保持不变。
