Golden J F, West S S, Shingleton H M, Můrad T M, Echols C K
J Histochem Cytochem. 1979 Jan;27(1):522-8. doi: 10.1177/27.1.374619.
A system for screening cervical cytological preparations is described which employs the Leitz Texture Analyzer System (E. Leitz, Rockleigh, N. J.) quantitative staining with acridine orange, and a fluorescence standard. The instrumentation scans cells on microscope slides and detects objects which it interprets to be nuclei with excess total nuclear green fluorescence intensity (Previous results employing manual measurements have indicated that normal nuclei do not produce total nuclear green fluorescence greater than a specific absolute intensity level). Detected objects are identified by visual observation. Cells (102,000) from 65 patients (29 normal, 36 abnormal) have been examined. In each abnormal sample, at least one abnormal cell was detected. In over half of the samples, three or fewer other objects (e.g. clumps of polymorphonuclear leukocytes) were detected. These are easily distinguishable from single nuclei, and could be discarded by someone with minimal cytological training.
本文描述了一种用于筛查宫颈细胞学涂片的系统，该系统采用徕卡纹理分析仪系统（新泽西州罗克利的徕卡公司），吖啶橙定量染色以及荧光标准。该仪器对显微镜载玻片上的细胞进行扫描，并检测其判定为具有过量总核绿色荧光强度的细胞核的物体（先前采用手动测量的结果表明，正常细胞核产生的总核绿色荧光不会大于特定的绝对强度水平）。通过目视观察识别检测到的物体。已对65名患者（29名正常，36名异常）的细胞（102,000个）进行了检查。在每个异常样本中，至少检测到一个异常细胞。在超过一半的样本中，检测到三个或更少的其他物体（例如多形核白细胞团块）。这些很容易与单个细胞核区分开来，并且经过最少细胞学培训的人员即可将其丢弃。
