Ganzhou Key Laboratory of Greenhouse Vegetable, School of Life Sciences, Gannan Normal University, Ganzhou 341000, China.
South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
J Agric Food Chem. 2023 Jul 26;71(29):10952-10969. doi: 10.1021/acs.jafc.3c03246. Epub 2023 Jul 18.
Brassica plants have glucosinolate (GLs)-myrosinase defense mechanisms to deter herbivores. However, specifically feeds on Brassica vegetables. The larvae possess three glucosinolate sulfatases (PxGSS1-3) that compete with plant myrosinase for shared GLs substrates and produce nontoxic desulfo-GLs (deGLs). Although PxGSSs are considered potential targets for pest control, the lack of a comprehensive review has hindered the development of PxGSSs-targeted pest control methods. Recent advances in integrative multi-omics analysis, substrate-enzyme kinetics, and molecular biological techniques have elucidated the evolutionary origin and functional diversity of these three PxGSSs. This review summarizes research progress on PxGSSs over the past 20 years, covering sequence properties, evolution, protein modification, enzyme activity, structural variation, substrate specificity, and interaction scenarios based on functional diversity. Finally, we discussed the potential applications of PxGSSs-targeted pest control technologies driven by artificial intelligence, including CRISPR/Cas9-mediated gene drive, transgenic plant-mediated RNAi, small-molecule inhibitors, and peptide inhibitors. These technologies have the potential to overcome current management challenges and promote the development and field application of PxGSSs-targeted pest control.
芸薹属植物具有含硫葡萄糖苷（GLs）-黑芥子酶防御机制来抵御食草动物。然而， specifically feeds on Brassica vegetables. 幼虫具有三种 GLs 磺基转移酶（PxGSS1-3），它们与植物黑芥子酶竞争共同的 GLs 底物，并产生无毒的脱磺 GLs（deGLs）。尽管 PxGSS 被认为是害虫防治的潜在目标，但缺乏全面的综述阻碍了基于 PxGSS 的害虫防治方法的发展。整合多组学分析、底物-酶动力学和分子生物学技术的最新进展阐明了这三种 PxGSS 的进化起源和功能多样性。本综述总结了过去 20 年来关于 PxGSS 的研究进展，涵盖了序列特性、进化、蛋白质修饰、酶活性、结构变异、底物特异性以及基于功能多样性的相互作用情况。最后，我们讨论了人工智能驱动的 PxGSS 靶向害虫防治技术的潜在应用，包括 CRISPR/Cas9 介导的基因驱动、转基因植物介导的 RNAi、小分子抑制剂和肽抑制剂。这些技术有可能克服当前的管理挑战，促进 PxGSS 靶向害虫防治的发展和田间应用。
