Phillips W D, Everett A W, Bennett M R
J Neurocytol. 1986 Jun;15(3):397-405. doi: 10.1007/BF01611441.
Many avian muscles contain a characteristic topographical distribution of fibre types. In order to study the role of nerves in the establishment and distribution of these fibre types, monoclonal antibodies (McAb) to the heavy chain subunit of myosin (MHC) were produced. The anti-fast McAb (2B12) bound to adult fast MHC and cross-reacted with the embryonic isoform of MHC. The anti-slow McAb (3D1) bound specifically to the heavy chain of slow myosin 2. By indirect immunofluorescence, anti-fast (2B12) stained all myotubes in the anterior latissimus dorsi and triceps and biceps muscles at stage 37 (11 days embryonic), whilst anti-slow (3D1) staining was largely restricted to the future slow fibres of these muscles. Brachial levels of the neural tube were surgically removed at stage 12 (2 days embryonic) so that muscles developed aneurally. Muscles at aneural stage 37 were smaller than normal, but the distribution of myotube types was not altered; all myotubes present still stained with anti-fast antibody while anti-slow staining was restricted to the anterior latissimus dorsi and myotubes in the deep parts of the triceps brachii pars scapularis, triceps brachii par humeralis and biceps brachii muscles (the future slow fibres of normal muscles). The results suggest that despite an overall reduction in MHC in aneural muscles, specialized fast and slow primary myotubes arise independently of the nerve in appropriate regions of the muscle.
许多鸟类肌肉含有纤维类型的特征性拓扑分布。为了研究神经在这些纤维类型的建立和分布中的作用，制备了针对肌球蛋白重链亚基（MHC）的单克隆抗体（McAb）。抗快肌单克隆抗体（2B12）与成年快肌MHC结合，并与MHC的胚胎异构体发生交叉反应。抗慢肌单克隆抗体（3D1）特异性结合慢肌球蛋白2的重链。通过间接免疫荧光法，抗快肌抗体（2B12）在第37阶段（胚胎11天）对背阔肌前部、肱三头肌和肱二头肌中的所有肌管进行了染色，而抗慢肌抗体（3D1）的染色主要局限于这些肌肉中未来的慢肌纤维。在第12阶段（胚胎2天）手术切除神经管的臂部水平，以使肌肉在无神经的情况下发育。无神经阶段37的肌肉比正常肌肉小，但肌管类型的分布没有改变；所有存在的肌管仍然用抗快肌抗体染色，而抗慢肌染色局限于背阔肌前部以及肱三头肌肩胛部、肱三头肌肱骨部和肱二头肌深部的肌管（正常肌肉中未来的慢肌纤维）。结果表明，尽管无神经肌肉中的MHC总体减少，但特化的快肌和慢肌初级肌管在肌肉的适当区域独立于神经产生。
