Nan Daneeya Na, Everts Vincent, Ferreira Joao N, Trachoo Vorapat, Osathanon Thanaphum, Klincumhom Nuttha, Pavasant Prasit
Center of Excellence in Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
BDJ Open. 2023 Jul 18;9(1):31. doi: 10.1038/s41405-023-00155-7.
The aim of this study was to investigate the effect of mechanical force on possible dynamic changes of the matrix proteins deposition in the PDL upon in vitro mechanical and in vivo occlusal forces in a rat model with hypofunctional conditions.
Intermittent compressive force (ICF) and shear force (SF) were applied to human periodontal ligament stem cells (PDLSCs). Protein expression of collagen I and POSTN was analyzed by western blot technique. To establish an in vivo model, rat maxillary molars were extracted to facilitate hypofunction of the periodontal ligament (PDL) tissue of the opposing mandibular molar. The mandibles were collected after 4-, 8-, and 12-weeks post-extraction and used for micro-CT and immunohistochemical analysis.
ICF and SF increased the synthesis of POSTN by human PDLSCs. Histological changes in the hypofunctional teeth revealed a narrowing of the PDL space, along with a decreased amount of collagen I, POSTN, and laminin in perivascular structures compared to the functional contralateral molars.
Our results revealed that loss of occlusal force disrupts deposition of some major matrix proteins in the PDL, underscoring the relevance of mechanical forces in maintaining periodontal tissue homeostasis by modulating ECM composition.
本研究旨在探讨在功能减退条件下的大鼠模型中，体外机械力和体内咬合力对牙周膜（PDL）中基质蛋白沉积可能产生的动态变化的影响。
对人牙周膜干细胞（PDLSCs）施加间歇性压缩力（ICF）和剪切力（SF）。采用蛋白质免疫印迹技术分析I型胶原蛋白和POSTN的蛋白表达。为建立体内模型，拔除大鼠上颌磨牙以促使对侧下颌磨牙的牙周膜（PDL）组织功能减退。在拔牙后4周、8周和12周收集下颌骨，用于显微CT和免疫组织化学分析。
ICF和SF增加了人PDLSCs中POSTN的合成。功能减退牙齿的组织学变化显示，与对侧功能正常的磨牙相比，PDL间隙变窄，血管周围结构中的I型胶原蛋白、POSTN和层粘连蛋白含量减少。
我们的结果表明，咬合力丧失会破坏PDL中一些主要基质蛋白的沉积，强调了机械力通过调节细胞外基质组成在维持牙周组织稳态中的相关性。
