Department of Neurology, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, No.136 Zhongshan 2nd Road, Yu Zhong District, Chongqing, 400014, China.
Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.
Exp Brain Res. 2023 Aug;241(8):2097-2106. doi: 10.1007/s00221-023-06663-0. Epub 2023 Jul 18.
To determine the dynamic effects of miR-20a-5p on hippocampal ripple energy in rats after status epilepticus (SE). A lithium pilocarpine (LiCl-PILO)-induced rat model of status epilepticus (SE) was established, and the rats were divided into the normal control (Control, CTL), epileptic control (PILO), valproic acid (VPA + PILO), miR-20a-5p overexpression lentivirus vector (miR + PILO), sponges blocking lentivirus vector (Sponges + PILO), and scramble sequence negative control (Scramble + PILO) groups (n = 6). Electroencephalograms (EEGs) were used to analyze changes in hippocampal ripple energy before and after SE. Quantitative polymerase chain reaction (q-PCR) analysis showed that miR-20a-5p levels gradually increased after miR-20a-5p overexpression lentivirus vector injection into the lateral ventricle, and the miR-20a-5p levels were significantly higher than that in CTL group on days 7 and 36 (P < 0.001). The miR-20a-5p levels decreased significantly on days 7 and 36 after blocking by sponges lentivirus vector injected into the lateral ventricle (P < 0.001). After injection of PILO, the average ripple energy expression in each group gradually increased, and reached the peak before chloral hydrate injection (compared with 1 day before SE, P < 0.05). The ripple energy in the VPA + PILO and Sponges + PILO groups was significantly lower than that in the PILO group at 60 min and 70 min after PILO injection and before chloral hydrate injection (P < 0.05), and maintained lower until 2 h after chloral hydrate injection in VPA + PILO (P < 0.05). Compared with the VPA + PILO group, the mean ripple energy of the Sponges + PILO group had no difference at all time points (P ≥ 0.05). After SE, ripple distribution of space and energy is closely related to the occurrence of epilepsy. Inhibition of miR20a-5p expression can downregulate ripple oscillation energy during seizure.
为了确定 miR-20a-5p 在癫痫持续状态（SE）后对大鼠海马回波能量的动态影响。建立锂匹罗卡品（LiCl-PILO）诱导的大鼠 SE 模型，将大鼠分为正常对照组（CTL）、癫痫对照组（PILO）、丙戊酸钠（VPA+PILO）、miR-20a-5p 过表达慢病毒载体（miR+PILO）、海绵体阻断慢病毒载体（Sponges+PILO）和 scramble 序列阴性对照（Scramble+PILO）组（n=6）。脑电图（EEG）用于分析 SE 前后海马回波能量的变化。实时定量聚合酶链反应（q-PCR）分析显示，miR-20a-5p 过表达慢病毒载体注入侧脑室后，miR-20a-5p 水平逐渐升高，第 7 天和第 36 天 miR-20a-5p 水平明显高于 CTL 组（P<0.001）。侧脑室注射海绵体慢病毒载体阻断后，miR-20a-5p 水平显著降低第 7 天和第 36 天（P<0.001）。PILO 注射后，各组平均波纹能量表达逐渐增加，在氯醛注射前达到峰值（与 SE 前 1 天相比，P<0.05）。VPA+PILO 和 Sponges+PILO 组的波纹能量在 PILO 注射后 60min 和 70min 及氯醛注射前明显低于 PILO 组（P<0.05），氯醛注射后 2h 仍维持在 VPA+PILO 组（P<0.05）。与 VPA+PILO 组相比，Sponges+PILO 组在所有时间点的平均波纹能量均无差异（P≥0.05）。SE 后，空间和能量的波纹分布与癫痫的发生密切相关。抑制 miR20a-5p 的表达可下调癫痫发作期间的波纹振荡能量。
