Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Methods Mol Biol. 2023;2677:269-280. doi: 10.1007/978-1-0716-3259-8_16.
Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of the pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. By modulating the FGF, TGF-β, and WNT pathways, we have derived intermediate PSCs (FTW-PSCs) that are permissive for direct primordial germ cell-like cell (PGC-LC) induction in vitro. Here, we describe the method for derivation and maintenance of mouse and human FTW-PSCs, as well as PGC-LC induction from FTW-PSCs.
动态多能干细胞（PSC）状态是体内多能性连续统的体外适应。先前的研究已经产生了许多具有不同特性的 PSC。通过调节 FGF、TGF-β 和 WNT 途径，我们已经衍生出了中间 PSC（FTW-PSC），它们在体外允许直接诱导原始生殖细胞样细胞（PGC-LC）。在这里，我们描述了从鼠和人 FTW-PSC 中诱导和维持 PGC-LC 的方法。
