Jin Danjie, Liu Ruiping, Xu Nanwei
Department of Orthopaedics, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu, China.
Orthop J Sports Med. 2023 Jul 13;11(7):23259671231178009. doi: 10.1177/23259671231178009. eCollection 2023 Jul.
Ghrelin, an amino acid hormone secreted primarily from the stomach, can regulate bone metabolism, regulate inflammation via suppressing proinflammatory cytokines, and suppress expression of matrix metalloproteinases (MMPs).
To measure synovial fluid levels of ghrelin in young patients with anterior cruciate ligament (ACL) tear to assess the role of ghrelin as a potential biomarker for cartilage injury.
Controlled laboratory study.
This study included 120 patients who underwent ACL reconstructionbetween January 1, 2016, and May 31, 2021. We categorized 60 patients with acute cartilage injury (International Cartilage Regeneration & Joint Preservation Society grade 2 or 3) as the acute group and 60 patients with no acute cartilage injury as the nonacute group, with the healthy contralateral knee of each patient acting as the control group (n = 120). Synovial fluid samples were collected from the knees in the operating room before ACL reconstruction. We assessed the inflammatory biomarkers interleukin (IL)-6, MMP-1, MMP-9, and MMP-13, as well as serum ghrelin level and Mankin score, and results were compared between the 3 study groups with the Mann-Whitney test.
Lower serum ghrelin levels in the synovial fluid were found in the acute group compared with the nonacute group and healthy controls (232.4 vs 434.4 vs 421.5 pg/mL, respectively; < .001). Ghrelin level in the synovial fluid was significantly and positively correlated with IL-6 ( = 0.4223; < .0001), MMP-13 ( = 0.3402; < .0001), and Mankin score ( = 0.1453; = .0244).
In patients with ACL injury, ghrelin synovial fluid was significantly differently expressed in patients with cartilage injury and no cartilage injury.
Ghrelin synovial fluid has the potential to be a biomarker to predict acute cartilage injury in patients with ACL injury.
胃饥饿素是一种主要由胃分泌的氨基酸激素，可调节骨代谢，通过抑制促炎细胞因子来调节炎症，并抑制基质金属蛋白酶（MMPs）的表达。
测量前交叉韧带（ACL）撕裂的年轻患者滑液中胃饥饿素的水平，以评估胃饥饿素作为软骨损伤潜在生物标志物的作用。
对照实验室研究。
本研究纳入了2016年1月1日至2021年5月31日期间接受ACL重建的120例患者。我们将60例急性软骨损伤患者（国际软骨再生与关节保护学会2级或3级）分为急性组，60例无急性软骨损伤患者分为非急性组，每位患者的健侧膝关节作为对照组（n = 120）。在ACL重建术前于手术室采集膝关节滑液样本。我们评估了炎症生物标志物白细胞介素（IL）-6、MMP-1、MMP-9和MMP-13，以及血清胃饥饿素水平和曼金评分，并使用曼-惠特尼检验比较了3个研究组之间的结果。
与非急性组和健康对照组相比，急性组滑液中血清胃饥饿素水平较低（分别为232.4 vs 434.4 vs 421.5 pg/mL；P <.001）。滑液中胃饥饿素水平与IL-6（r = 0.4223；P <.0001）、MMP-13（r = 0.3402；P <.0001）和曼金评分（r = 0.1453；P =.0244）显著正相关。
在ACL损伤患者中，软骨损伤患者和无软骨损伤患者的滑液胃饥饿素表达存在显著差异。
滑液胃饥饿素有可能成为预测ACL损伤患者急性软骨损伤的生物标志物。
