De Virgilio Armando, Veneroni Maria Vittoria, Costantino Andrea, Festa Bianca Maria, Fiamengo Barbara, Sebastiani Daniela, Spriano Giuseppe, Di Tommaso Luca
Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, MI, Italy.
Otorhinolaryngology Unit, IRCCS Humanitas Research Hospital, Rozzano, MI, Italy.
Front Med (Lausanne). 2023 Jul 3;10:1163565. doi: 10.3389/fmed.2023.1163565. eCollection 2023.
The purpose of this study is to define if tumor-infiltrating lymphocytes (TILs) and tumor-associated macrophages (TAMs) could represent potential predictors of lymph node metastases (LNM) in salivary gland cancers (SGC).
A selected number of immunohistochemical markers related to TILs (CD3, CD4, CD68, and FOXP3) and TAMs (CD68 and CD163) were investigated on major salivary gland cancers. TIL and TAM densities were measured on digital images using the open-source QuPath both in the tumor interior (TI) and invasive margin (IM). Correlation with pathologic N classification and follow-up clinical data was investigated.
A total of 25 consecutive patients (men: 11; median age: 62.0) were included. Densities of CD3+ IM (OR = 7.7, 95% CI 1.2-51.2), CD8+ TI (OR = 7.7, 95% CI 1.2-51.2), CD8+ IM (OR = 7.7, 95% CI 1.2-51.2), FOXP3+ TI (OR = 24.0, 95% CI 2.2-255.9), CD68+ TI (OR = 7.7, 95% CI 1.2-51.2), and CD163+ IM (OR = 7.7, 95% CI 1.2 - 51.2), and the Immunoscore CD8/CD3 (OR = 1.9, 95% CI 1.1-3.4) were significantly associated with LNM ( < 0.05). CD3+ TI density was significantly associated with tumor recurrence and death (HR = 5.8, 95% CI 1.5-22.6; < 0.05).
A high density of specific TIL and TAM subpopulations might be correlated with a higher probability of LNM in SGC.
本研究旨在确定肿瘤浸润淋巴细胞（TILs）和肿瘤相关巨噬细胞（TAMs）是否可作为唾液腺癌（SGC）淋巴结转移（LNM）的潜在预测指标。
对所选的一些与TILs（CD3、CD4、CD68和FOXP3）和TAMs（CD68和CD163）相关的免疫组化标志物在大唾液腺癌中进行研究。使用开源的QuPath在数字图像上测量肿瘤内部（TI）和浸润边缘（IM）的TIL和TAM密度。研究其与病理N分类及随访临床数据的相关性。
共纳入25例连续患者（男性11例；中位年龄62.0岁）。CD3⁺IM密度（OR = 7.7，95%CI 1.2 - 51.2）、CD8⁺TI密度（OR = 7.7，95%CI 1.2 - 51.2）、CD8⁺IM密度（OR = 7.7，95%CI 1.2 - 51.2）、FOXP3⁺TI密度（OR = 24.0，95%CI 2.2 - 255.9）、CD68⁺TI密度（OR = 7.7，95%CI 1.2 - 51.2）和CD163⁺IM密度（OR = 7.7，95%CI 1.2 - 51.2）以及免疫评分CD8/CD3（OR = 1.9，95%CI 1.1 - 3.4）与LNM显著相关（P < 0.05）。CD3⁺TI密度与肿瘤复发和死亡显著相关（HR = 5.8，95%CI 1.5 - 22.6；P < 0.05）。
特定TIL和TAM亚群的高密度可能与SGC中LNM的较高概率相关。
