Department of Neurology, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, No. 43 Renmin Avenue, 570208, Haikou, Hainan, China.
An Acad Bras Cienc. 2023 Jul 17;95(suppl 1):e20220750. doi: 10.1590/0001-3765202320220750. eCollection 2023.
Sleep deprivation (SD) can lead to cognitive impairment caused by neuroinflammation. MiR-181c-5p/HMGB1 axis plays a part in anti-inflammation effects. However, the mechanism that miR-181c-5p facilitates learning and memory in SD mice remains unclear. So we investigated the role of miR-181c-5p in learning and memory impairment induced by SD. We overexpressed miR-181c-5p in the mice hippocampus by injecting lentivirus vector-miR-181c-5p (LV-miR-181c-5p) particles. Mice were divided into four groups: control (Ctrl), SD, SD + miR-181c-5p and SD + vector. We found that mice in the third group showed ameliorated learning and memory compared with the fourth group. The content of ionized calcium binding adaptor molecule 1 (IBA-1) in the third group was decreased compared with the fourth group. Moreover, the expression levels of HMGB1, TLR4 and p-NF-κB in the hippocampus of overexpressed miR-181c-5p mice were reduced. In total, miR-181c-5p ameliorated learning and memory in SD mice via the HMGB1/TLR4/NF-κB pathway.
睡眠剥夺（SD）可导致神经炎症引起的认知障碍。miR-181c-5p/HMGB1 轴在抗炎作用中发挥作用。然而，miR-181c-5p 促进 SD 小鼠学习和记忆的机制尚不清楚。因此，我们研究了 miR-181c-5p 在 SD 诱导的学习和记忆障碍中的作用。我们通过注射慢病毒载体-miR-181c-5p（LV-miR-181c-5p）颗粒在小鼠海马体中转染 miR-181c-5p。将小鼠分为四组：对照组（Ctrl）、SD 组、SD+miR-181c-5p 组和 SD+载体组。我们发现第三组小鼠的学习和记忆能力较第四组有所改善。第三组的离子钙结合接头分子 1（IBA-1）含量较第四组减少。此外，过表达 miR-181c-5p 小鼠海马体中 HMGB1、TLR4 和 p-NF-κB 的表达水平降低。总之，miR-181c-5p 通过 HMGB1/TLR4/NF-κB 通路改善了 SD 小鼠的学习和记忆能力。
