Key Laboratory of Molecular Biology and Genetic Engineering of Jiangxi Province, School of Life Sciences, Nanchang University, Nanchang 330031, China; Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Key Laboratory of Molecular Biology and Genetic Engineering of Jiangxi Province, School of Life Sciences, Nanchang University, Nanchang 330031, China.
Plant Sci. 2023 Oct;335:111798. doi: 10.1016/j.plantsci.2023.111798. Epub 2023 Jul 17.
Inositolphosphorylceramide synthase (IPCS) catalyses ceramides and phosphatidylinositol (PI) into inositolphosphorylceramide (IPC), which is involved in the regulation of plant growth and development. A total of three OsIPCS family genes have been identified in rice. However, most of their functions remain unknown. Here, the functions of OsIPCSs were analyzed by CRISPR/Cas9 technology, lipidomics analysis, and transcriptomics analysis. Single-gene mutation of OsIPCSs resulted in dwarf phenotype. Among them, the phenotype of osipcs3 mutant was more severe. Multi-gene mutation of OsIPCS genes led to more severe phenotypes, indicating the additive effects of OsIPCSs. We further determined that a significant decrease in epidermal cell elongation of internode in the mutants. There was a significant decrease in the content of IPC detected in the osipcs2/3 and osipcs1/2/3 mutants. The contents of glycosyl inositol phosphoryl ceramide (GIPC) were also decreased by 20% and 10% in osipcs2/3 and osipcs1/2/3, respectively. The results of RNA-seq showed that numerous DEGs found to be associated with cellular component organization, anatomical structure morphogenesis, and cell growth in the osipcs2, osipcs2/3, and osipcs1/2/3. Taken together, OsIPCSs may be involved in the regulation of plant height through affecting cell growth and sphingolipid metabolism in rice.
肌醇磷酸神经酰胺合酶（IPCS）催化神经酰胺和磷脂酰肌醇（PI）生成肌醇磷酸神经酰胺（IPC），参与植物生长发育的调控。在水稻中已鉴定出三种 OsIPCS 家族基因。然而，它们的大多数功能仍然未知。本研究利用 CRISPR/Cas9 技术、脂质组学分析和转录组学分析，对 OsIPCSs 的功能进行了分析。OsIPCSs 的单基因突变导致矮化表型。其中，osipcs3 突变体的表型更为严重。OsIPCS 基因的多基因突变导致更为严重的表型，表明 OsIPCSs 存在累加效应。我们进一步确定突变体中节间表皮细胞伸长显著减少。osipcs2/3 和 osipcs1/2/3 突变体中 IPC 的含量显著降低。osipcs2/3 和 osipcs1/2/3 突变体中糖基肌醇磷酸神经酰胺（GIPC）的含量也分别下降了 20%和 10%。RNA-seq 的结果表明，在 osipcs2、osipcs2/3 和 osipcs1/2/3 中发现了许多与细胞成分组织、解剖结构形态发生和细胞生长相关的 DEGs。综上所述，OsIPCSs 可能通过影响细胞生长和水稻中鞘脂代谢来参与株高的调控。
