Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand.
Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 755-8611, Japan.
Bioresour Technol. 2023 Oct;386:129519. doi: 10.1016/j.biortech.2023.129519. Epub 2023 Jul 17.
The transition to renewable energy sources is crucial to ensure a sustainable future. Although the sugar and ethanol industries benefit from this transition, there are untapped opportunities to utilize the waste generated from the sugar and ethanol process chains through two-stage anaerobic digestion (TSAD). This review comprehensively discusses the utilization of various sugarcane-based industrial wastes by TSAD for sequential biohydrogen and methane production. Factors influencing TSAD process performance, including pH, temperature, hydraulic retention time, volatile fatty acids and alkalinity, nutrient imbalance, microbial population, and inhibitors, were discussed in detail. The potential of TSAD to reduce emissions of greenhouse gases is demonstrated. Recent findings, implications, and promising future research related to TSAD, including the integration of meta-omics approaches, gene manipulation and bioaugmentation, and application of artificial intelligence, are highlighted. The review can serve as important literature for the implementation, improvement, and advancements in TSAD research.
向可再生能源的转型对于确保可持续的未来至关重要。尽管糖和乙醇产业从这一转型中受益，但通过两步厌氧消化（TSAD）利用糖和乙醇加工链中产生的废物仍有未开发的机会。本综述全面讨论了通过 TSAD 利用各种基于甘蔗的工业废物进行连续生物氢气和甲烷生产。详细讨论了影响 TSAD 工艺性能的因素，包括 pH 值、温度、水力停留时间、挥发性脂肪酸和碱度、营养失衡、微生物种群和抑制剂。还展示了 TSAD 减少温室气体排放的潜力。强调了与 TSAD 相关的最新发现、意义和有前途的未来研究，包括宏基因组学方法的整合、基因操作和生物增强以及人工智能的应用。该综述可作为实施、改进和推进 TSAD 研究的重要文献。
