Forss-Petter S, Danielson P, Sutcliffe J G
J Neurosci Res. 1986;16(1):141-56. doi: 10.1002/jnr.490160114.
The protein encoded by a randomly selected rat brain cDNA clone was identified as neuron-specific enolase (NSE; 4.2.1.11; gamma subunit), based on homology to yeast enolase sequences and the presence of the corresponding 2.5-kb mRNA in rat brain but not in liver, kidney, or muscle tissue. The 2,222-nucleotide NSE and mRNA sequence presented identifies a 68-nucleotide 5' noncoding region, a 1,302-nucleotide open reading frame (corresponding to a primary translation product of 434 amino acids), and 852 noncoding 3' bases. Evolutionary implications based on sequence comparisons to yeast enolase and non-neuronal enolase are discussed. Primer extension analysis indicated the presence of several alternative initiation sites for transcription within 60 nucleotides on the NSE gene. The developmental onset of NSE mRNA expression correlates with the appearance of NSE protein; however, the mRNA reaches adult levels by postnatal week 3, whereas the protein continues to accumulate over the next few months, suggesting regulatory mechanisms in addition to transcriptional control.
根据与酵母烯醇化酶序列的同源性以及大鼠脑中存在相应的2.5 kb mRNA，而肝、肾或肌肉组织中不存在该mRNA，一个随机选择的大鼠脑cDNA克隆编码的蛋白质被鉴定为神经元特异性烯醇化酶（NSE；4.2.1.11；γ亚基）。所呈现的2222个核苷酸的NSE和mRNA序列确定了一个68个核苷酸的5'非编码区、一个1302个核苷酸的开放阅读框（对应于434个氨基酸的初级翻译产物）以及852个非编码3'碱基。讨论了基于与酵母烯醇化酶和非神经元烯醇化酶序列比较的进化意义。引物延伸分析表明，NSE基因上60个核苷酸内存在几个转录起始位点的替代位点。NSE mRNA表达的发育起始与NSE蛋白的出现相关；然而，mRNA在出生后第3周达到成年水平，而蛋白质在接下来的几个月中继续积累，这表明除了转录控制外还存在调节机制。
