脑区 CA2 区的 CaMKIIα 神经元调控小鼠的注意行为。

CaMKIIα neurons in the anterior insular cortex regulate attention behavior in mice.

机构信息

School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, China.

Department of Neurobiology, Beijing Institute of Basic Medical Sciences, Beijing, China.

出版信息

Front Neural Circuits. 2023 Jul 4;17:1197541. doi: 10.3389/fncir.2023.1197541. eCollection 2023.

DOI:10.3389/fncir.2023.1197541

PMID:37469833

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10352765/

Abstract

INTRODUCTION

The insular cortex is involved in multiple physiological processes including working memory, pain, emotion, and interoceptive functions. Previous studies have indicated that the anterior insular cortex (aIC) also mediates interoceptive attention in humans. However, the exact cellular and physiological function of the aIC in the regulation of this process is still elusive.

METHODS

In this study, using the 5-choice serial reaction time task (5-CSRTT) testing paradigm, we assessed the role of the aIC in visuospatial attention and impulsiveness in mice.

RESULTS

The results showed a dramatic activation of c-Fos in the aIC CaMKIIα neurons after the 5-CSRTT procedure. fiber photometry revealed enhanced calcium signaling in aIC CaMKIIα neurons when the mice responded correctly. In addition, chemogenetic suppression of aIC CaMKIIα neurons led to increased incorrect responses within the appropriate time. Importantly, pharmacological activation of aIC CaMKIIα neurons enhanced their performance in the 5-CSRTT test.

DISCUSSION

These results provide compelling evidence that aIC CaMKIIα neurons are essential for the modulation of attentional processing in mice.

摘要

简介

脑岛皮层参与多种生理过程，包括工作记忆、疼痛、情绪和内脏感觉功能。先前的研究表明，前脑岛皮层（aIC）也介导了人类的内脏感觉注意。然而，aIC 在调节这一过程中的确切细胞和生理功能仍不清楚。

方法

在这项研究中，我们使用 5 选择序列反应时间任务（5-CSRTT）测试范式，评估了 aIC 在小鼠视觉空间注意力和冲动性中的作用。

结果

结果显示，在 5-CSRTT 程序后，aIC CaMKIIα 神经元中的 c-Fos 显著激活。光纤光度法显示，当小鼠正确反应时，aIC CaMKIIα 神经元中的钙信号增强。此外，化学遗传抑制 aIC CaMKIIα 神经元会导致在适当时间内增加错误反应。重要的是，药理学激活 aIC CaMKIIα 神经元增强了它们在 5-CSRTT 测试中的表现。