Pulse techniques for the suppression of individual components in multiexponential relaxation curves.

NMR pulse sequences have been investigated which selectively suppress undesired signal components from multiple-exponential spin-spin and spin-lattice relaxation curves. Suppression is achieved by exploiting differences in the relaxation times of sample components. Special attention is devoted to discussion of practical details necessary for implementation of these methods, as well as their relative advantages and disadvantages. The techniques are illustrated using multiple-compartment aqueous phantoms and are then employed in measurements of proton T1 and T2 values for freshly isolated guinea pig lenses immersed in aqueous buffer solutions to prevent dehydration and maintain viability. These methods obviate many of the difficulties inherent in obtaining accurate relaxation times for minor components of multiple-exponential curves and should be useful in many applications of NMR to the study of intact tissue, both in vivo and in vitro.