Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, PR China.
South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15;303:123149. doi: 10.1016/j.saa.2023.123149. Epub 2023 Jul 14.
In this work, a novel "turn-on" fluorescence sensor for the detection of HO and glucose was developed based on green fluorescent carbon dots (CDs). The CDs was newly prepared by a facile one-pot hydrothermal method with Eosin Y and branched polyethylenimine as precursors. Interestingly, in the presence of HO and HRP, the fluorescence of the CDs enhanced significantly with a red-shift emission due to their "aggregation". Meanwhile, the oxidation of glucose catalyzed by glucose oxidase could generate HO. Thus, a simple sensing system based on the CDs as fluorescent probes was constructed for HO and glucose determination, avoiding the fluorescence quenching and subsequent recovery process in conventional turn-on strategy. The method showed good selectivity and sensitivity for glucose sensing with the detection limit of 0.12 μM. The method was further applied to glucose detection in real samples. The obtained results demonstrated the simplicity, selectivity and practicality of the method. This work expands the carbon nanomaterials with fluorescence emission enhancement properties. It provides a new and direct "turn-on" strategy for HO and glucose detection, which could be a simple and effective tool for screening biological substances involved in HO-generation reaction.
在这项工作中，我们基于绿色荧光碳点（CDs）开发了一种用于检测 HO 和葡萄糖的新型“开启”荧光传感器。该 CDs 是通过简单的一锅水热法，以曙红 Y 和支化聚乙烯亚胺为前体制备的。有趣的是，在存在 HO 和 HRP 的情况下，由于其“聚集”，CDs 的荧光显著增强，并伴有红移发射。同时，葡萄糖氧化酶催化的葡萄糖氧化可以产生 HO。因此，构建了基于 CDs 作为荧光探针的简单传感系统，用于 HO 和葡萄糖的测定，避免了传统“开启”策略中荧光猝灭和随后的恢复过程。该方法对葡萄糖检测具有良好的选择性和灵敏度，检测限为 0.12 μM。该方法进一步应用于实际样品中的葡萄糖检测。所得结果表明该方法具有简单、选择性和实用性。这项工作扩展了具有荧光增强性能的碳纳米材料。它为 HO 和葡萄糖检测提供了一种新的直接“开启”策略，可能成为筛选涉及 HO 生成反应的生物物质的简单有效工具。
