Foliar application of green synthesized ZnO nanoparticles reduced Cd content in shoot of lettuce.

Though Zinc (Zn) supplementation can mitigate root-based Cadmium (Cd) uptake in plants, the impact of foliar-applied Zinc Oxide nanoparticles (ZnO NPs) on this process remains under-explored. This study investigates the influence of foliar-applied ZnO NPs on the growth of lettuce and its Cd uptake in Cd-contaminated soil in greenhouse setting. Green synthesized ZnO (G-ZnO) NPs (10 and 100 mg/L) using sweet potato leaf extracts were used, and compared with commercially available ZnO (C-ZnO) NPs (100 mg/L) for their efficacy. Scanning electron microscopy and Fourier-transform infrared spectroscopy were used for G-ZnO NPs characterization. Shoot dry weight, antioxidant activity, and chlorophyll content were all negatively affected by Cd but positively affected by ZnO NPs application. ZnO NPs application resulted in a notable reduction in lettuce Cd uptake, with the highest reduction (43%) observed at 100 mg/L G-ZnO NPs. In the lettuce shoot, Zn and Cd concentration showed a significant inverse correlation (R = 0.79-0.9, P < 0.05). This study offers insights into the impact of chemical and green synthesized ZnO NPs on enhancing crop growth under stress conditions, and their role in modulating Cd uptake in plants, indicating potential implications for sustainable agricultural practices.