Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Science, 8815713471 Shahrekord, Iran; Cancer Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran 1417614411, Iran.
Int J Biol Macromol. 2023 Nov 1;251:125898. doi: 10.1016/j.ijbiomac.2023.125898. Epub 2023 Jul 20.
An optimal tissue-engineered dermal substitute should possess biocompatibility and cell adhesion conduction to facilitate fibroblast and keratinocyte infiltration and proliferation, as well as angiogenesis potential to escalate wound healing. Zinc was doped to bioactive glass-ceramic (Zn-BGC) to promote biocompatibility and angiogenesis properties. Zn-BGC was then incorporated into a collagen (Col) and gelatin (Gel) porous scaffold. The bioactive porous bionanocomposite exhibited biocompatibility along with improved cell attachment and proliferation. Scaffolds including Col-Gel/Zn-BGC with or without mouse embryonic fibroblasts were applied on full-thickness skin wounds on the BALB/c mice to assess their wound healing potential in vivo. The results indicated that the biodegradation rate of the Col-Gel/Zn-BGC nanocomposites was comparable to the rate of skin tissue regeneration in vivo. Macroscopic wound healing results showed that Col-Gel/Zn-BGC loaded with mouse embryonic fibroblast possesses the smallest wound size, indicating the fastest healing process. Histopathological evaluations displayed that the optimal wound regeneration was observed in Col-Gel/Zn-BGC nanocomposites loaded with mouse embryonic fibroblasts indicated by epithelialization and angiogenesis; besides the number of fibroblasts and hair follicles was increased. The bioactive nanocomposite scaffold of Col-Gel containing Zn-BGC nanoparticles loaded with mouse embryonic fibroblasts can be employed as a desirable skin substitute to ameliorate cutaneous wound regeneration.
一种理想的组织工程皮肤替代物应具有生物相容性和细胞附着传导性，以促进成纤维细胞和角质形成细胞的渗透和增殖，以及血管生成潜力，以加速伤口愈合。锌被掺杂到生物活性玻璃陶瓷（Zn-BGC）中以提高生物相容性和血管生成特性。然后将 Zn-BGC 掺入到胶原（Col）和明胶（Gel）多孔支架中。这种生物活性多孔仿生纳米复合材料表现出良好的生物相容性，同时改善了细胞附着和增殖。将包含 Col-Gel/Zn-BGC 的支架（有或没有小鼠胚胎成纤维细胞）应用于 BALB/c 小鼠的全层皮肤伤口上，以评估它们在体内的伤口愈合潜力。结果表明，Col-Gel/Zn-BGC 纳米复合材料的生物降解率与体内皮肤组织再生的速度相当。宏观伤口愈合结果表明，负载有小鼠胚胎成纤维细胞的 Col-Gel/Zn-BGC 具有最小的伤口尺寸，表明愈合过程最快。组织病理学评估显示，在负载有小鼠胚胎成纤维细胞的 Col-Gel/Zn-BGC 纳米复合材料中观察到最佳的伤口再生，表现为上皮化和血管生成；此外，成纤维细胞和毛囊的数量增加。负载有小鼠胚胎成纤维细胞的含 Zn-BGC 纳米颗粒的 Col-Gel 生物活性纳米复合支架可作为一种理想的皮肤替代物，改善皮肤伤口再生。
