Department of Chemistry and Chemical Biology, Rutgers, The State University of New, Jersey, Piscataway, NJ 08854, United States.
Department of Chemistry and Chemical Biology, Rutgers, The State University of New, Jersey, Piscataway, NJ 08854, United States.
J Magn Reson. 2023 Sep;354:107524. doi: 10.1016/j.jmr.2023.107524. Epub 2023 Jul 15.
The advent of magic angle spinning (MAS) rates exceeding 100 kHz has facilitated the acquisition of H-detected solid-state NMR spectra of biomolecules with high resolution. However, challenges can arise when preparing rotors for these experiments, due to the physical properties of biomolecular solid samples and the small dimensions of the rotors. In this study, we have designed 3D-printable centrifugal devices that facilitate efficient and consistent packing of crystalline protein slurries or viscous phospholipids into 0.7 mm rotors. We demonstrate the efficacy of these packing devices using H-detected solid state NMR at 105 kHz. In addition to devices for 0.7 mm rotors, we have also developed devices for other frequently employed rotor sizes and styles. We have made all our designs openly accessible, and we encourage their usage and ongoing development as a shared effort within the solid state NMR community.
魔角旋转(MAS)速率超过 100 kHz 的出现促进了具有高分辨率的生物分子的 H 检测固态 NMR 光谱的获取。然而,由于生物分子固体样品的物理性质和转子的小尺寸,在为这些实验准备转子时可能会出现挑战。在这项研究中,我们设计了 3D 可打印的离心装置,可将结晶蛋白悬浮液或粘性磷脂有效地、一致地填充到 0.7 mm 的转子中。我们使用 105 kHz 的 H 检测固态 NMR 来证明这些包装装置的功效。除了用于 0.7 mm 转子的装置外,我们还开发了用于其他常用转子尺寸和样式的装置。我们已经公开了所有的设计,并鼓励在固态 NMR 社区中共同使用和不断开发。