Böckmann Anja, Ernst Matthias, Meier Beat H
Institut de Biologie et Chimie des Protéines, BMSSI, UMR 5086 CNRS/Université de Lyon 1, 7 passage du Vercors, 69367 Lyon, France.
Physical Chemistry, ETH Zurich, Vladimir-Prelog-Weg 2, 8093 Zurich, Switzerland.
J Magn Reson. 2015 Apr;253:71-9. doi: 10.1016/j.jmr.2015.01.012.
Magic-angle spinning (MAS) is a technique that is a prerequisite for high-resolution solid-state NMR spectroscopy of proteins and other biomolecules. Recently, the 100 kHz limit for the rotation frequency has been broken, arguably making MAS rotors the man-made objects with the highest rotation frequency. This development is expected to have a significant impact on biomolecular NMR as it facilitates proton detection, which allows to partially compensate the loss in overall sensitivity associated with the small sample amounts that fit into MAS rotors with less than 1 mm outer diameter. Under these conditions, the mass-normalized sensitivity of a small rotor becomes much higher than that of larger-volume rotor.
魔角旋转（MAS）是蛋白质和其他生物分子高分辨率固态核磁共振光谱的一项必备技术。最近，旋转频率的100千赫兹限制已被突破，可以说使MAS转子成为旋转频率最高的人造物体。这一进展预计将对生物分子核磁共振产生重大影响，因为它有助于质子检测，这可以部分补偿与装入外径小于1毫米的MAS转子中的少量样品相关的整体灵敏度损失。在这些条件下，小转子的质量归一化灵敏度比大体积转子的灵敏度高得多。
