Department of Community Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Community Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand; School of Integrative Medicine, Mae Fah Luang University, Chiang Rai 57100, Thailand.
J Infect Public Health. 2023 Sep;16(9):1418-1426. doi: 10.1016/j.jiph.2023.07.009. Epub 2023 Jul 20.
Human respiratory syncytial virus (hRSV) is an important cause of acute respiratory infection, especially in children. Few studies have investigated molecular epidemiology of hRSV infection in Thailand. The aims of this study were to investigate the prevalence and genotype diversity of hRSV in children with acute respiratory infection (ARI) in Thailand.
A total of 383 nasopharyngeal swabs collected from children with ARI from October 2020 to September 2021 were screened for hRSV and nucleotide sequences of the hypervariable region 2 (HVR2) of G gene of the detected hRSV were analysed.
Of 383 nasopharyngeal swabs, 104 (27.2 %) were positive for hRSV, of which 51 (49.0 %), 43 (41.3 %), and 10 (9.6 %) were hRSV-A, hRSV-B, and untypeable strains, respectively. All hRSV-A and hRSV-B were ON1 genotype and BA9 genotype, respectively. Most of the hRSV strains were detected in the cool months, November 2020 to February 2021. Phylogenetic analysis of the HVR2 sequence of G gene revealed three clusters of hRSV-A (ON1 genotype) and two clusters of hRSV-B (BA9 genotype). The hRSV-A strains in cluster 1 and 3 were closely related to the hRSV-A reference strains reported previously from other regions of Thailand whereas those in cluster 2 were closely related to the hRSV-A reference strains reported previously from Europe and Africa. For the hRSV-B strains, both clusters 1 and 2 were closely related to the hRSV-B reference strains reported previously from Europe, Australia, and Taiwan. The predicted N- and O-linked glycosylation sites were found along the length of HVR2 of G protein, mostly in the hRSV-B strains.
The ON1 and BA9 were the only two hRSV genotypes that were co-predominant and solely detected in this study. The findings indicated that the ON1 and BA9 are the only two hRSV genotypes currently circulating in children with ARI in northern Thailand.
人类呼吸道合胞病毒（hRSV）是急性呼吸道感染的重要原因，尤其是在儿童中。泰国对 hRSV 感染的分子流行病学研究较少。本研究旨在调查泰国急性呼吸道感染（ARI）患儿 hRSV 的流行率和基因型多样性。
2020 年 10 月至 2021 年 9 月，共采集 383 份鼻咽拭子，筛查 hRSV，对检出的 hRSV G 基因高变区 2（HVR2）核苷酸序列进行分析。
383 份鼻咽拭子中，104 份（27.2%）hRSV 阳性，其中 hRSV-A、hRSV-B 和未定型株分别为 51 株（49.0%）、43 株（41.3%）和 10 株（9.6%）。所有 hRSV-A 和 hRSV-B 均为 ON1 基因型和 BA9 基因型。大多数 hRSV 株在凉爽月份（2020 年 11 月至 2021 年 2 月）检出。G 基因 HVR2 序列的系统进化分析显示，hRSV-A（ON1 基因型）有 3 个聚类，hRSV-B（BA9 基因型）有 2 个聚类。聚类 1 和 3 的 hRSV-A 株与泰国其他地区先前报道的 hRSV-A 参考株密切相关，而聚类 2 的 hRSV-A 株与欧洲和非洲先前报道的 hRSV-A 参考株密切相关。对于 hRSV-B 株，聚类 1 和 2 与欧洲、澳大利亚和中国台湾地区先前报道的 hRSV-B 参考株密切相关。在 G 蛋白 HVR2 全长上发现了 N-和 O-连接的糖基化位点，主要存在于 hRSV-B 株中。
ON1 和 BA9 是本研究中唯一共同占优势且仅检测到的两种 hRSV 基因型。研究结果表明，ON1 和 BA9 是目前泰国北部 ARI 患儿中流行的两种 hRSV 基因型。
