Zou L R, Li Z C, Zhong Z F, Liang L J, Song Y C, Wu J
Institute of Pathogenic Microbiology, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2020 Mar 6;54(3):294-300. doi: 10.3760/cma.j.issn.0253-9624.2020.03.010.
To understand the genetic variation and epidemiological characteristics of human respiratory syncytial virus (HRSV) in Guangzhou. Nasopharyngeal swabs specimens were collected from 0-6 year old children hospitalized with acute respiratory infection, then HRSV was tested and genotyped by RT-PCR. Phylogenetic tree was bulit using MEGA 6.0 software. NetNGlyc 1.0 server was used to predict the potential N-linked glycosylation sites. A total of 1 225 nasopharyngeal specimens were collected, including 783 males and 442 females. The median ((25), (75)) age was 8 (3, 24) months. Among the 209 HRSV-positive cases (17.06%), 117 cases (55.98%) were HRSV-A and 92 cases (44.02%) were HRSV-B. The two distinct subgroups (HRSV-A and HRSV-B) alternately played dominant role to cause HRSV infection and exchange almost once every two years. The HRSV prevalence rate decreased with age. The HRSV-positive rate among children under 2 years old was 18.83% (196 cases), accounting for 93.78% of the total positive cases. There were 32 HRSV positive cases co-infected with at least one respiratory virus, with the co-infection rate of 15.31%. Phylogenetic tree analysis of the second hypervariable region (HVR2) of the G protein classified the HRSV-A specimens into ON1 (62) and NA1 (2) genotypes while all HRSV-B specimens belonged to BA genotype (53). The HVR2 of the G protein varied in using stop condon, amino acid substitutions, glycosylation sites. Children under 2 years old were the high risk population of HRSV infection in Guangzhou. ON1 genotype turned into a primary genetype of the HRSV-A subgroup while BA genotype dominated the HRSV-B subgroup. A greater diversification of amino acid substitutions, and some deletion and insertion of glycosylation sites embodied the polymorphism of G protein as main protective antigen.
为了解广州地区人呼吸道合胞病毒（HRSV）的基因变异情况及流行病学特征。收集0 - 6岁因急性呼吸道感染住院儿童的鼻咽拭子标本，采用逆转录聚合酶链反应（RT-PCR）对HRSV进行检测和基因分型。使用MEGA 6.0软件构建系统发育树。利用NetNGlyc 1.0服务器预测潜在的N-糖基化位点。共收集1225份鼻咽标本，其中男性783例，女性442例。年龄中位数（（25），（75））为8（3，24）个月。在209例HRSV阳性病例（17.06%）中，117例（55.98%）为HRSV-A型，92例（44.02%）为HRSV-B型。两个不同的亚组（HRSV-A和HRSV-B）交替发挥主导作用导致HRSV感染，且几乎每两年交替一次。HRSV感染率随年龄增长而降低。2岁以下儿童HRSV阳性率为18.83%（196例），占总阳性病例的93.78%。有32例HRSV阳性病例合并感染至少一种呼吸道病毒，合并感染率为15.31%。对G蛋白第二个高变区（HVR2）的系统发育树分析将HRSV-A标本分为ON1（62）和NA1（2）基因型，而所有HRSV-B标本均属于BA基因型（53）。G蛋白的HVR2在终止密码子使用、氨基酸替换、糖基化位点方面存在差异。2岁以下儿童是广州地区HRSV感染的高危人群。ON1基因型成为HRSV-A亚组的主要基因型，而BA基因型在HRSV-B亚组中占主导地位。氨基酸替换的更大差异以及糖基化位点的一些缺失和插入体现了作为主要保护性抗原的G蛋白的多态性。
