Department of Medical Biochemistry, Kafrelsheikh University,Egypt.
Department of Medical Biochemistry,Egypt.
J Pak Med Assoc. 2023 Apr;73(Suppl 4)(4):S184-S190. doi: 10.47391/JPMA.EGY-S4-37.
To assess the serum expression levels of long non-coding ribonucleic acid growth arrest specific-5 and micro-ribonucleic acid-137, and the different genotypes of long non-coding ribonucleic acid growth arrestspecific-5 rs2067079 (C>T) and micro-ribonucleic acid-137 rs1625579 (T>G) in acute ischaemic stroke patients.
The case-control study was conducted at Cairo University, Cairo, Egypt, from January to August 2020, and comprised adult acute ischaemic stroke patients of either gender selected from the stroke unit of the Neurology Department at Kasr Alainy Hospital of Cairo University. Healthy individuals matched for age and gender were enrolled as controls. Quantitative real-time polymerase chain reaction was used to quantify serum expression levels of long non-coding ribonucleic acid growth arrest specific-5 and micro-ribonucleic acid-137, and to genotype long non coding ribonucleic acid lncRNA growth arrest specific-5 rs2067079 and micro-ribonucleic acid-137 rs1625579 using TaqMan allelic discrimination. Data was analysed using SPSS 22.
Of the 100 subjects, 50(50%) were patients; 34(68%) males and 16(32%) females with mean age 60.4±10.0 years. The remaining 50(50%) were controls; 28(56%) males and 22(44%) females with mean age 56.9±12.2 years (p>0.05). The patients had more smokers, more hypertensives and more diabetics than the controls (p<0.05). Long non-coding ribonucleic acid growth arrest specific-5 expression levels were significantly increased, while microribonucleic acid-137 expression levels were significantly reduced among the patients(p<0.05). Acute ischaemic stroke risk was significantly higher in patients with growth arrest specific-5 rs2067079 (C>T) recessive model (homozygous minor TT genotype), while micro-ribonucleic acid-137 rs1625579 (T>G) was protective against acute ischaemic stroke in allelic (G minor allele), codominant (GT genotype), dominant (GT+GG), and over-dominant (GT genotype) models (p<0.05).
Long non-coding ribonucleic acid growth arrest specific-5 rs2067079 and micro-ribonucleic acid-137 rs1625579 may act as novel genetic markers of acute ischaemic stroke risk.
评估长链非编码核糖核酸生长停滞特异性 5 和 micro-ribonucleic 酸 137 的血清表达水平，以及长链非编码核糖核酸生长停滞特异性 5 rs2067079（C>T）和 micro-ribonucleic 酸 137 rs1625579（T>G）的不同基因型在急性缺血性脑卒中患者中的表达。
本病例对照研究于 2020 年 1 月至 8 月在埃及开罗大学进行，纳入了来自开罗大学卡西阿兰尼医院神经科卒中单元的成年急性缺血性脑卒中患者作为病例，并按年龄和性别与健康个体进行匹配作为对照。采用实时定量聚合酶链反应检测长链非编码核糖核酸生长停滞特异性 5 和 micro-ribonucleic 酸 137 的血清表达水平，并采用 TaqMan 等位基因鉴别法对长链非编码核糖核酸 lncRNA 生长停滞特异性 5 rs2067079 和 micro-ribonucleic 酸 137 rs1625579 进行基因分型。数据分析采用 SPSS 22 软件。
100 例患者中，50 例（50%）为患者，34 例（68%）为男性，16 例（32%）为女性，平均年龄 60.4±10.0 岁。其余 50 例（50%）为对照组，28 例（56%）为男性，22 例（44%）为女性，平均年龄 56.9±12.2 岁（p>0.05）。与对照组相比，患者中吸烟者、高血压患者和糖尿病患者更多（p<0.05）。患者的长链非编码核糖核酸生长停滞特异性 5 表达水平明显升高，而 micro-ribonucleic 酸 137 表达水平明显降低（p<0.05）。生长停滞特异性 5 rs2067079（C>T）隐性模型（纯合子 TT 基因型）患者的急性缺血性脑卒中风险显著升高，而 micro-ribonucleic 酸 137 rs1625579（T>G）在等位基因（G 小等位基因）、共显性（GT 基因型）、显性（GT+GG）和超显性（GT 基因型）模型中对急性缺血性脑卒中具有保护作用（p<0.05）。
长链非编码核糖核酸生长停滞特异性 5 rs2067079 和 micro-ribonucleic 酸 137 rs1625579 可能是急性缺血性脑卒中风险的新型遗传标志物。
