Ferrer I, Fábregues I, Rairiz J, Galofré E
Neurosci Lett. 1986 Aug 15;69(1):115-9. doi: 10.1016/0304-3940(86)90425-8.
Samples of the cerebral cortex (left hemisphere, area 6) were collected at autopsy, between 4 and 6 h after death in order to avoid artifacts related to fixation delay, from 5 chronic alcoholic patients (in which well-defined alcoholic-malnutritional encephalopathies were excluded) and 16 controls; samples were immediately processed according to the rapid Golgi method. The curves representing the average density of dendritic spines on the apical dendrites of layer V pyramidal neurons as a function of the distance from the cell body were adjusted to a logarithmical model which was shown to be similar in controls and alcoholic groups. Significant reduced numbers of dendritic spines were observed; however, in the alcoholic patients when compared to age-matched controls (P less than 0.001; Kruskall-Wallis chi 2 test). These results demonstrate noxious effects of ethanol on cortical pyramidal neurons in human chronic alcoholism.
为避免与固定延迟相关的假象，在5名慢性酒精中毒患者（排除明确的酒精性营养不良性脑病患者）和16名对照者死亡后4至6小时进行尸检时，采集大脑皮层样本（左半球，6区）；样本立即按照快速高尔基方法进行处理。将代表V层锥体神经元顶树突上树突棘平均密度随距细胞体距离变化的曲线拟合为对数模型，结果显示该模型在对照组和酒精组中相似。观察到树突棘数量显著减少；然而，与年龄匹配的对照组相比，酒精中毒患者的树突棘数量显著减少（P<0.001；克鲁斯卡尔 - 沃利斯卡方检验）。这些结果表明，乙醇对人类慢性酒精中毒患者的皮层锥体神经元具有有害作用。
