Rawson Kaden B, Neuberger Travis, Smith Tyler B, Bell Isaac J, Looper Ryan E, Sebahar Paul R, Haussener Travis J, Kanna Reddy Hariprasada Reddy, Isaacson Brad M, Shero John, Pasquina Paul F, Williams Dustin L
Department of Orthopaedics, University of Utah, Salt Lake City, UT, USA.
Bone and Biofilm Research Lab, University of Utah, Salt Lake City, UT, USA.
Biofilm. 2023 Jul 11;6:100142. doi: 10.1016/j.bioflm.2023.100142. eCollection 2023 Dec 15.
Implementation of negative pressure wound therapy (NPWT) as a standard of care has proven efficacious in reducing both the healing time and likelihood of nosocomial infection among pressure ulcers and traumatic, combat-related injuries. However, current formulations may not target or dramatically reduce bacterial biofilm burden following therapy. The purpose of this study was to determine the antibiofilm efficacy of an open-cell polyurethane (PU) foam (V.A.C.® Granufoam™) loaded with a first-in-class compound (CZ-01179) as the active release agent integrated via lyophilized hydrogel scaffolding. An porcine excision wound model was designed to perform antibiofilm efficacy testing in the presence of NPWT. PU foam samples loaded with a 10.0% w/w formulation of CZ-01179 and 0.5% hyaluronic acid were prepared and tested against current standards of care: V.A.C.® Granufoam Silver™ and V.A.C.® Granufoam™. We observed statistically significant reduction of methicillin-resistant (MRSA) and biofilms with the CZ-01179 antibiofilm foam in comparison to current standard of care foams. These findings motivate further development of an antibiofilm PU foam loaded with CZ-01179.
负压伤口治疗（NPWT）作为一种标准治疗方法，已被证明在减少压疮以及创伤性、与战斗相关损伤的愈合时间和医院感染可能性方面是有效的。然而，目前的配方可能无法在治疗后靶向或显著降低细菌生物膜负荷。本研究的目的是确定一种开孔聚氨酯（PU）泡沫（V.A.C.® Granufoam™）的抗生物膜功效，该泡沫负载了一种一流的化合物（CZ-01179）作为通过冻干水凝胶支架整合的活性释放剂。设计了一个猪切除伤口模型，以在NPWT存在的情况下进行抗生物膜功效测试。制备了负载10.0% w/w CZ-01179和0.5%透明质酸配方的PU泡沫样品，并与当前的护理标准：V.A.C.® Granufoam Silver™和V.A.C.® Granufoam™进行测试。与当前护理标准泡沫相比，我们观察到CZ-01179抗生物膜泡沫对耐甲氧西林金黄色葡萄球菌（MRSA）和生物膜有统计学上的显著减少。这些发现推动了对负载CZ-01179的抗生物膜PU泡沫的进一步开发。
