Stocker F, Herschkowitz N, Bossi E, Stoller M, Cross T A, Aue W P, Seelig J
Pediatr Res. 1986 Sep;20(9):867-71. doi: 10.1203/00006450-198609000-00012.
Cerebral high energy phosphates were studied in the intact rabbit brain using nuclear magnetic resonance spectroscopy. The effect of hypothermia on degradation kinetics in total ischemia due to circulatory arrest was examined, measuring phosphocreatine, adenosine triphosphate, and inorganic phosphate as a function of time at three different temperatures (35, 24, 21 degrees C). Phosphocreatine- and ATP-decays followed single exponential functions at all three temperatures. The half-life times increased by approximately a factor of three upon lowering the temperature from 35 to 21 degrees C with activation energies of 15-20 kcal/mol, which corresponds to values of Q10 between 2.4 and 3.2. In the temperature range studied, no critical temperature was found below which metabolism would stop completely. We conclude that nuclear magnetic resonance spectroscopy allows, in the intact animal, quantitative assessment of the influence of hypothermia on energy metabolism in the brain. This influence is a major concern in the field of cardiac surgery in infants and children who are often operated in total circulatory arrest under deep hypothermia.
利用核磁共振波谱法对完整兔脑的脑高能磷酸盐进行了研究。研究了低温对因循环骤停导致的完全缺血时降解动力学的影响，在三个不同温度（35、24、21℃）下测量磷酸肌酸、三磷酸腺苷和无机磷酸盐随时间的变化。在所有三个温度下，磷酸肌酸和三磷酸腺苷的衰减均遵循单指数函数。将温度从35℃降至21℃时，半衰期增加了约三倍，活化能为15 - 20千卡/摩尔，这对应于2.4至3.2之间的Q10值。在所研究的温度范围内，未发现代谢会完全停止的临界温度。我们得出结论，核磁共振波谱法能够在完整动物中对低温对脑能量代谢的影响进行定量评估。这种影响是婴幼儿心脏手术领域的一个主要关注点，这些患儿常在深度低温下进行全循环骤停手术。
