Natural and Medical Sciences Research Center, University of Nizwa, Nizwa 616, Oman.
Department of Biological Sciences and Chemistry, Faculty of Arts and Sciences, University of Nizwa, Nizwa 616, Oman.
Int Immunopharmacol. 2023 Sep;122:110668. doi: 10.1016/j.intimp.2023.110668. Epub 2023 Jul 22.
Boswellic acids have been recognized as anti-inflammatory and immunomodulatory agents with potentials to control autoimmune and inflammatory diseases. However, their effects on T cell proliferation and activation are not fully elucidated. In this study, we investigated effects of individual compounds including β-Boswellic acids (β-BA), 11-keto-β-Boswellic acid (β-KBA), 3-O-acetyl β-Boswellic acids (β-ABA), and 3-O-acetyl-11-keto-β-Boswellic acid (β-AKBA) on human peripheral blood mononuclear cells (PBMCs) and their potential role in modulating immune responses. We showed that β-BA, KBA, and AKBA at a 0.025 µM concentration significantly reduced T cell proliferation without inducing cytotoxicity, however, ABA showed cytotoxic effects at this concentration. β-BA and KBA showed significantly reduced T cell proliferation at 0.05 µM concentration without cytotoxic effects. Interestingly, we found that AKBA at 0.025 µM concentration significantly reduced CD25 expression on both CD4 and CD8 T cells without cytotoxic effects. Additionally, β-BA reduced CD25 expression on both CD4 and CD8 T cells at 0.05 µM concentration with no cytotoxicity. In this study, we determined the optimum concentration of each of these compounds that have the potential to reduce T cell activation without cytotoxic effects. Our findings show that both β-BA and AKBA have the ability to inhibit T cell proliferation and activation without inducing cytotoxicity. Further investigations are required to fully understand the mechanisms underlying these effects and the potential therapeutic benefits of these compounds in different autoimmune and inflammatory settings.
乳香酸已被公认为具有抗炎和免疫调节作用的药物，有可能控制自身免疫和炎症性疾病。然而，其对 T 细胞增殖和活化的影响尚未完全阐明。在这项研究中，我们研究了包括β-乳香酸（β-BA）、11-酮-β-乳香酸（β-KBA）、3-O-乙酰基-β-乳香酸（β-ABA）和 3-O-乙酰基-11-酮-β-乳香酸（β-AKBA）在内的单一化合物对人外周血单核细胞（PBMCs）的影响及其在调节免疫反应中的潜在作用。我们发现，β-BA、KBA 和 AKBA 在 0.025µM 浓度下可显著抑制 T 细胞增殖而不诱导细胞毒性，而 ABA 在该浓度下则显示出细胞毒性作用。β-BA 和 KBA 在 0.05µM 浓度下可显著抑制 T 细胞增殖而不产生细胞毒性。有趣的是，我们发现 AKBA 在 0.025µM 浓度下可显著降低 CD4 和 CD8 T 细胞上 CD25 的表达而不产生细胞毒性。此外，β-BA 在 0.05µM 浓度下可降低 CD4 和 CD8 T 细胞上 CD25 的表达而不产生细胞毒性。在这项研究中，我们确定了这些化合物具有降低 T 细胞激活而无细胞毒性的最佳浓度。我们的研究结果表明，β-BA 和 AKBA 均具有抑制 T 细胞增殖和活化而不诱导细胞毒性的能力。需要进一步的研究来充分了解这些作用的机制以及这些化合物在不同的自身免疫和炎症环境中的潜在治疗益处。
