State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, 100871, China; PKU-IDG/McGovern Institute for Brain Research, Beijing, 100871, China.
State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, 100871, China; PKU-IDG/McGovern Institute for Brain Research, Beijing, 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China.
Curr Opin Neurobiol. 2023 Aug;81:102751. doi: 10.1016/j.conb.2023.102751. Epub 2023 Jul 22.
Neuropeptides comprise the most diverse category of neurochemicals in the brain, playing critical roles in a wide range of physiological and pathophysiological processes. Monitoring neuropeptides with high spatial and temporal resolution is essential for understanding how peptidergic transmission is regulated throughout the central nervous system. In this review, we provide an overview of current non-optical and optical approaches used to detect neuropeptides, including their design principles, intrinsic properties, and potential limitations. We also highlight the advantages of using G protein‒coupled receptor (GPCR) activation‒based (GRAB) sensors to monitor neuropeptides in vivo with high sensitivity, good specificity, and high spatiotemporal resolution. Finally, we present a promising outlook regarding the development and optimization of new GRAB neuropeptide sensors, as well as their potential applications.
神经肽是大脑中种类最多的神经化学物质，在广泛的生理和病理生理过程中发挥着关键作用。 以高时空分辨率监测神经肽对于了解肽能传递如何在整个中枢神经系统中受到调节至关重要。 在这篇综述中，我们概述了目前用于检测神经肽的非光学和光学方法，包括它们的设计原理、固有特性和潜在的局限性。 我们还强调了使用 G 蛋白偶联受体 (GPCR) 激活基 (GRAB) 传感器监测体内神经肽的高灵敏度、良好的特异性和高时空分辨率的优势。 最后，我们对新型 GRAB 神经肽传感器的开发和优化以及它们的潜在应用提出了有希望的展望。
