Division of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine Budapest, István Street 2, H-1078 Budapest, Hungary; National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine Budapest, István Street 2, H-1078, Hungary.
Division of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine Budapest, István Street 2, H-1078 Budapest, Hungary.
Insect Biochem Mol Biol. 2023 Aug;159:103990. doi: 10.1016/j.ibmb.2023.103990. Epub 2023 Jul 23.
Excessive use of azole fungicides in agriculture poses a potential threat to honeybees and other pollinator insects; however, the detailed effects of these molecules remain largely unclear. Hence, in the present study it was aimed to investigate the acute sublethal effects of tebuconazole on the redox homeostasis and fatty acid composition in the brain of honeybees. Our findings demonstrate that tebuconazole decreased total antioxidant capacity, the ratio of reduced to oxidized glutathione and disturbed the function of key antioxidant defense enzymes along with the induction of lipid peroxidation indicated by increased malondialdehyde levels, while it also altered the fatty acid profile of the brain. The present study highlights the negative impact of tebuconazole on honeybees and contributes to the understanding of potential consequences related to azole exposure on pollinator insects' health, such as the occurrence of colony collapse disorder.
农业中过度使用唑类杀菌剂对蜜蜂和其他传粉昆虫构成潜在威胁；然而，这些分子的详细影响在很大程度上仍不清楚。因此，本研究旨在调查唑醚菌酯对蜜蜂大脑氧化还原稳态和脂肪酸组成的急性亚致死作用。我们的研究结果表明，唑醚菌酯降低了总抗氧化能力、还原型谷胱甘肽与氧化型谷胱甘肽的比值，并干扰了关键抗氧化防御酶的功能，同时还诱导了脂质过氧化，表现为丙二醛水平升高，而大脑的脂肪酸谱也发生了改变。本研究强调了唑醚菌酯对蜜蜂的负面影响，并有助于了解与授粉昆虫健康相关的唑类暴露的潜在后果，例如蜂群崩溃失调的发生。
