Laboratory of Virology and Rickettsial Infections, Veterinary Hospital, Federal University of Mato Grosso, Cuiabá, Brazil.
Statistics Department, Federal University of Mato Grosso, Cuiabá, Brazil.
Vet Clin Pathol. 2023 Dec;52(4):621-627. doi: 10.1111/vcp.13277. Epub 2023 Jul 24.
Synthetic peptides of tandem repeat proteins (TRPs) have been employed in the serologic analysis of canine monocytic ehrlichiosis (CME) and used in epidemiological studies in Brazil. Based on molecular studies of TRPs, different genotypes of Ehrlichia canis have been described, but data on their pathogenicity remain unknown.
To correlate hepatic, renal, and muscular alterations in relation to different genotypes of E. canis in naturally exposed dogs using enzyme-linked immunosorbent assay (ELISA) with TRP19 and TRP36 synthetic protein antigens.
Two hundred serum samples were subjected to ELISA with the antigens of TRP19 and three genotypes (US, Br, and CR) of TRP36 of E. canis circulating in Brazil. Positive sera were evaluated through eight biochemical parameters, and the results were evaluated by principal component analysis and canonical correlation.
ELISA revealed that 47 (23.5%) serum samples reacted to the BrTRP36 peptide, 36 (18%) reacted to the TRP19 peptide, and 8 (4%) reacted to the USTRP36 and CRTRP36 peptides separately. The most frequent biochemical alterations observed were for CK (59.4%), ALB (31.8%), GLO (28.9%), TP (28.9%), ALP (26%), urea (24.6%), creatinine (14.4%), and ALT (14.4%). The most prominent diagnostic method in canonical correlation analysis was BrTRP36, followed by TRP19, which correlated with hyperglobulinemia and hypoalbuminemia.
Antibodies that reacted against the Brazilian genotype of E. canis correlated positively with hyperglobulinemia and increases in serum urea and creatinine. According to our results, the Brazilian genotype of E. canis is related to the chronic phase of CME.
串联重复蛋白（TRP）的合成肽已被用于犬单核埃立克体病（CME）的血清学分析，并用于巴西的流行病学研究。基于 TRP 的分子研究，已描述了不同基因型的犬埃立克体，但关于其致病性的数据仍不清楚。
使用 ELISA 结合 TRP19 和 TRP36 合成蛋白抗原，与巴西流行的 E. canis 的不同基因型相关联，以确定肝、肾和肌肉的改变。
用 ELISA 检测 200 份血清样本，抗原为 TRP19 和 E. canis 的三种基因型（US、Br 和 CR）的 TRP36。阳性血清通过八项生化参数进行评估，并通过主成分分析和典型相关进行结果评估。
ELISA 显示 47 份（23.5%）血清样本对 BrTRP36 肽反应，36 份（18%）对 TRP19 肽反应，8 份（4%）分别对 USTRP36 和 CRTRP36 肽反应。观察到最常见的生化改变是 CK（59.4%）、ALB（31.8%）、GLO（28.9%）、TP（28.9%）、ALP（26%）、尿素（24.6%）、肌酐（14.4%）和 ALT（14.4%）。在典型相关分析中，最突出的诊断方法是 BrTRP36，其次是 TRP19，与高球蛋白血症和低白蛋白血症相关。
针对巴西基因型 E. canis 的抗体与高球蛋白血症和血清尿素、肌酐增加呈正相关。根据我们的结果，巴西基因型 E. canis 与 CME 的慢性期有关。
