Institute of Bioscience and BioResources, National Research Council, Via Università 100, 80055, Portici, Italy.
Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055, Portici, Italy.
Planta. 2023 Jul 24;258(3):50. doi: 10.1007/s00425-023-04200-5.
DcMYB11, an R2R3 MYB gene associated with petiole anthocyanin pigmentation in carrot, was functionally characterized. A putative enhancer sequence is able to increase DcMYB11 activity. The accumulation of anthocyanin pigments can exhibit different patterns across plant tissues and crop varieties. This variability allowed the investigation of the molecular mechanisms behind the biosynthesis of these pigments in several plant species. Among crops, carrots have a well-defined anthocyanin pigmentation pattern depending on the genic background. In this work, we report on the discovery of DNA structural differences affecting the activity of an R2R3 MYB (encoded by DcMYB11) involved in anthocyanin regulation in carrot petiole. To this end, we first verified the function of DcMYB11 using heterologous systems and identified three different alleles which may explain differences in petiole pigmentation. Characterization of the DcMYB11 alleles at the 5' upstream sequence unveiled a sequence that functions as a putative enhancer. In conclusion, this study provides novel insight into the molecular mechanisms controlling anthocyanin accumulation in carrot. By these outcomes, we expanded our knowledge on the cis-regulatory sequences in plants.
DcMYB11，一个与胡萝卜叶柄花青素色素沉着相关的 R2R3 MYB 基因，其功能特征得到了研究。一个假定的增强子序列能够增加 DcMYB11 的活性。花青素色素的积累在植物组织和作物品种中表现出不同的模式。这种可变性允许对几种植物物种中这些色素生物合成的分子机制进行研究。在作物中，胡萝卜的花青素色素沉着模式取决于基因背景。在这项工作中，我们报告了发现影响参与胡萝卜叶柄花青素调控的 R2R3 MYB（由 DcMYB11 编码）活性的 DNA 结构差异。为此，我们首先使用异源系统验证了 DcMYB11 的功能，并鉴定了三个不同的等位基因，这些等位基因可能解释了叶柄色素沉着的差异。对 5'上游序列的 DcMYB11 等位基因的表征揭示了一个作为假定增强子的序列。总之，本研究为控制胡萝卜中花青素积累的分子机制提供了新的见解。通过这些结果，我们扩展了我们对植物顺式调控序列的知识。
