Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Department of Urology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
IET Syst Biol. 2023 Oct;17(5):245-258. doi: 10.1049/syb2.12073. Epub 2023 Jul 24.
The progression of prostate cancer (PCa) leads to poor prognosis. However, the molecular mechanism of PCa is still not completely clear. This study aimed to elucidate the important role of centromere protein A (CENPA) in PCa. Large numbers of bulk RNA sequencing (RNA-seq) data and in-house immunohistochemistry data were used in analysing the expression level of CENPA in PCa and metastatic PCa (MPCa). Single-cell RNA-seq data was used to explore the expression status of CENPA in different prostate subpopulations. Enrichment analysis was employed to detect the function of CENPA in PCa. Clinicopathological parameters analysis was utilised in analysing the clinical value of CENPA. The results showed that CENPA was upregulated in PCa (standardised mean difference [SMD] = 0.83, p = 0.001) and MPCa (SMD = 0.61, p = 0.029). CENPA was overexpressed in prostate cancer stem cells (CSCs) with androgen receptor (AR) negative compared to epithelial cells with AR positive. CENPA may influence the development of PCa through affecting cell cycle. Patients with nodal metastasis had higher expression level of CENPA. And patients with high CENPA expression had poor disease-free survival. Taken together, Overexpression of CENPA may influence the development of PCa by regulating cell cycle and promoting metastasis.
前列腺癌（PCa）的进展导致预后不良。然而，PCa 的分子机制仍不完全清楚。本研究旨在阐明着丝粒蛋白 A（CENPA）在 PCa 中的重要作用。大量批量 RNA 测序（RNA-seq）数据和内部免疫组织化学数据用于分析 PCa 和转移性 PCa（MPCa）中 CENPA 的表达水平。单细胞 RNA-seq 数据用于探索 CENPA 在不同前列腺亚群中的表达状态。富集分析用于检测 CENPA 在 PCa 中的功能。临床病理参数分析用于分析 CENPA 的临床价值。结果表明，CENPA 在 PCa（标准化均数差 [SMD] = 0.83，p = 0.001）和 MPCa（SMD = 0.61，p = 0.029）中上调。与 AR 阳性的上皮细胞相比，具有 AR 阴性的前列腺癌干细胞（CSCs）中 CENPA 过表达。CENPA 可能通过影响细胞周期影响 PCa 的发展。有淋巴结转移的患者 CENPA 表达水平更高。CENPA 高表达的患者无病生存期较差。总之，CENPA 的过表达可能通过调节细胞周期和促进转移来影响 PCa 的发展。
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