Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Department of Biological Sciences, College of Basic and Applied Sciences, Mountain Top University Ibafo, Ogun State, Nigeria.
Theriogenology. 2023 Oct 15;210:101-109. doi: 10.1016/j.theriogenology.2023.07.013. Epub 2023 Jul 13.
LIF is crucial in regulating embryo implantation, while HOXA10 is a marker gene for uterine receptivity. However, the specific mechanism of LIF regulating HOXA10 during cow embryo implantation has not been fully understood. To address this knowledge gap, the experiment involved treating bovine endometrial epithelial cells (BEECs) with LIF to investigate the relationship between LIF, miRNA, and HOXA10. The experimental findings revealed that applying LIF resulted in a substantial increase in the proliferation of endometrial epithelial cells. Moreover, the expressions of PI3K, AKT, HOXA10, CDK4, cyclinD1, and cyclinE1 were significantly elevated. Conversely, the expression of p21Cipl was significantly reduced. In the group that received a combination of LIF and a STAT3 inhibitor, the expression of PI3K/AKT remained significantly increased, but there was no significant change in the expression of HOXA10. When miRNA-27a-3p was overexpressed, it resulted in a decrease in both the RNA and protein expression of HOXA10. Conversely, inhibiting miRNA-27a-3p increased the RNA and protein expression of HOXA10. In the presence of LIF treatment, the expression of miRNA-27a-3p was reduced, while the expression of HOXA10 was increased. However, when LIF and a STAT3 inhibitor were combined, there was no significant change in the expression of miRNA-27a-3p or HOXA10. Consequently, LIF facilitated cell proliferation by activating the PI3K/AKT pathway. LIF controlled the expression of miRNA-27a-3p and HOXA10 in endometrial epithelial cells through STAT3, with miRNA-27a-3p negatively regulating the expression of HOXA10.
LIF 在调节胚胎着床中起着至关重要的作用，而 HOXA10 是子宫容受性的标记基因。然而，LIF 调节牛胚胎着床过程中 HOXA10 的具体机制尚未完全阐明。为了解决这一知识空白，该实验涉及用 LIF 处理牛子宫内膜上皮细胞（BEEC），以研究 LIF、miRNA 和 HOXA10 之间的关系。实验结果表明，应用 LIF 可显著促进子宫内膜上皮细胞的增殖。此外，PI3K、AKT、HOXA10、CDK4、cyclinD1 和 cyclinE1 的表达显著升高。相反，p21Cipl 的表达显著降低。在接受 LIF 和 STAT3 抑制剂联合处理的组中，PI3K/AKT 的表达仍然显著增加，但 HOXA10 的表达没有明显变化。当 miRNA-27a-3p 过表达时，HOXA10 的 RNA 和蛋白质表达均降低。相反，抑制 miRNA-27a-3p 增加了 HOXA10 的 RNA 和蛋白质表达。在 LIF 处理存在的情况下，miRNA-27a-3p 的表达减少，而 HOXA10 的表达增加。然而，当 LIF 和 STAT3 抑制剂联合使用时，miRNA-27a-3p 或 HOXA10 的表达没有显著变化。因此，LIF 通过激活 PI3K/AKT 通路促进细胞增殖。LIF 通过 STAT3 控制子宫内膜上皮细胞中 miRNA-27a-3p 和 HOXA10 的表达，miRNA-27a-3p 负调控 HOXA10 的表达。
