Post-graduation Program of Biochemistry and Molecular Biology, Laboratory of Toxicological Genetic, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Post-graduation Program of Development and Environment - DDMA, Federal University of Rio Grande do Norte (UFRN), Natal/RN, Brazil.
Mutat Res Genet Toxicol Environ Mutagen. 2023 Jul;889:503652. doi: 10.1016/j.mrgentox.2023.503652. Epub 2023 Jun 23.
Radon gas inhalation is the main source of exposure to ionizing radiation by humans. There is still lack in knowledge concerning the chronic and indirect effects of exposure to this carcinogenic factor. Therefore, the aim of this work is to analyze the levels of oxidative genomic damage in inhabitants of a medium-high background radiation area (HBRA) (N = 82) in Northeastern Brazil and compare them with people living in a low background radiation area (LBRA) (N = 46). 8-hydroxy-2-deoxyguanosine (8-OHdG) was quantified in urine, Ser326Cys polymorphism was determined in the hOGG1 gene and indoor radon was measured. HBRA houses had 6.5 times higher indoor radon levels than those from LBRA (p-value < 0.001). The 8-OHdG mean (95% confidence interval) were significantly different, 8.42 (5.98-11.9) ng/mg creatinine and 29.91 (23.37-38.30) ng/mg creatinine for LBRA and HBRA, respectively. The variables representing lifestyle and environmental and occupational exposures did not have a significant association with oxidized guanosine concentrations. On the other hand, lower 8-OHdG values were observed in subjects that had one mutant allele (326Cys) in the hOGG1 gene than those who had both wild alleles (Ser/Ser (p-value < 0.05). It can be concluded that high radon levels have significantly influenced the genome oxidative metabolism and hOGG1 gene polymorphism would mediate the observed biological response.
氡气吸入是人类暴露于电离辐射的主要来源。对于暴露于这种致癌因素的慢性和间接影响，人们的认识仍然不足。因此，本工作的目的是分析巴西东北部中高水平本底辐射区（HBRA）（N=82）居民的氧化基因组损伤水平，并将其与生活在低水平本底辐射区（LBRA）（N=46）的人进行比较。尿中定量了 8-羟基-2-脱氧鸟苷（8-OHdG），测定了 hOGG1 基因的 Ser326Cys 多态性，并测量了室内氡。HBRA 住宅的室内氡水平比 LBRA 住宅高 6.5 倍（p 值<0.001）。8-OHdG 的平均值（95%置信区间）差异显著，LBRA 和 HBRA 的 8-OHdG 分别为 8.42（5.98-11.9）ng/mg 肌酐和 29.91（23.37-38.30）ng/mg 肌酐。代表生活方式以及环境和职业暴露的变量与氧化鸟苷浓度没有显著相关性。另一方面，在 hOGG1 基因中具有一个突变等位基因（326Cys）的个体中，8-OHdG 值较低，而具有两个野生等位基因（Ser/Ser）的个体中 8-OHdG 值较高（p 值<0.05）。可以得出结论，高水平的氡显著影响了基因组的氧化代谢，而 hOGG1 基因多态性将介导观察到的生物学反应。
