Department of Advanced Oral Surgery, Kanagawa Dental University, Yokohama Clinic, 3-31-6 Tsuruya-cho, Kanagawa-ku, Yokohama City, Kanagawa, 221-0835, Japan.
Department of Environmental Pathology, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka City, Kanagawa, 238-8580, Japan.
J Stomatol Oral Maxillofac Surg. 2023 Dec;124(6S):101572. doi: 10.1016/j.jormas.2023.101572. Epub 2023 Jul 24.
Despite the role of transmembrane protease, serine 2 (TMPRSS2) in facilitating the entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the primary cause of the global COVID-19 pandemic, the interaction of extracellular and intracellular proteases in this process remains poorly elucidated. Thus, we monitored the salivary expression concentration (SEC) of TMPRSS2 and its inhibitor, alpha-1 antitrypsin (A1AT), and investigated whether oral inflammatory diseases affected the SEC of both proteins.
We collected saliva samples before and after surgical treatment of inflammatory cystic diseases (radicular and inflammatory dentigerous cysts) in 25 patients. The SEC of TMPRSS2 and A1AT was measured using enzyme-linked immunosorbent assay. SEC in multiple patient status groups and subgroups of each status were investigated. Finally, the correlation between TMPRSS2 and A1AT SEC was analyzed.
The TMPRSS2 and A1AT SEC did not significantly change pre- or post-treatment. The TMPRSS2 SEC was significantly higher before and after treatment in patients aged >50 years, patients with radicular cysts, and patients with the basic disease. A1AT SEC was significantly decreased after treatment in the acute inflammation, large-sized, and patients without basic disease groups. No significant correlation was observed between the SEC of either protein before and after treatment.
Individual-specific SEC for TMPRSS2 may be influenced by age, lesion type, and basic disease; however, oral inflammatory diseases may not have a direct effect. Moreover, the extent of oral inflammatory diseases and the presence of basic diseases may be associated with A1AT SEC. Furthermore, the SEC between the two proteins may be independent.
尽管跨膜丝氨酸蛋白酶 2（TMPRSS2）在促进严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的进入方面发挥作用，SARS-CoV-2 是导致全球 COVID-19 大流行的主要原因，但这一过程中外源和细胞内蛋白酶的相互作用仍不清楚。因此，我们监测了 TMPRSS2 及其抑制剂α-1 抗胰蛋白酶（A1AT）的唾液表达浓度（SEC），并研究了口腔炎性疾病是否会影响这两种蛋白的 SEC。
我们收集了 25 例接受炎性囊性疾病（根尖和炎性牙源性囊肿）手术治疗前后的唾液样本。采用酶联免疫吸附试验（ELISA）法检测 TMPRSS2 和 A1AT 的 SEC。研究了多个患者状态组和每个状态的亚组的 SEC，并分析了 TMPRSS2 和 A1AT SEC 之间的相关性。
治疗前后 TMPRSS2 和 A1AT SEC 均无显著变化。>50 岁的患者、根尖囊肿患者和基础疾病患者的 TMPRSS2 SEC 在治疗前后均显著升高。急性炎症、大尺寸和无基础疾病患者的 A1AT SEC 在治疗后显著降低。治疗前后两种蛋白的 SEC 之间无显著相关性。
TMPRSS2 的个体特异性 SEC 可能受年龄、病变类型和基础疾病的影响，但口腔炎性疾病可能没有直接影响。此外，口腔炎性疾病的严重程度和基础疾病的存在可能与 A1AT SEC 有关。此外，两种蛋白之间的 SEC 可能是独立的。
