Zhang Qi, Wu Shiman, Li Yajie, Lai Mao, Li Qing, Fu Caixia, Yao Zhenwei, Zhang Junhai
Department of Radiology, Huashan Hospital, Fudan University Shanghai 200040 P.R. China
Department of Radiology, The First People's Hospital of Jinghong Jinghong City Yunnan Province P.R. China.
Nanoscale Adv. 2023 Jun 21;5(15):3994-4001. doi: 10.1039/d2na00787h. eCollection 2023 Jul 25.
Endometriosis is a tumor-like disease with high recurrence. In this case, the accurate imaging-based diagnosis of endometriosis can help clinicians eradicate it by improving their surgical plan. However, although contrast agents can improve the visibility of the tissue of interest magnetic resonance imaging (MRI), the lack of biomarkers in endometriosis hinders the development of agents for its targeted imaging and diagnosis. Herein, aiming at the enriched vascular endothelial growth factor (VEGF) in endometriosis, we developed a targeting MRI contrast agent modified with bevacizumab, , NaGdF@PEG@bevacizumab-Cy5.5 nanoparticles (NPBCNs), to detect endometriosis. NPBCNs showed negligible cytotoxicity and high affinity towards VEGF in endometrial cells . Furthermore, NPBCNs generated a strong signal enhancement in endometriosis lesions in rats in -weighted images MRI at 3 days post-injection, as confirmed by the histopathological staining results and fluorescence imaging on the same day. Our approach can enable NPBCNs to target endometriosis effectively, thus avoiding missed diagnoses.
子宫内膜异位症是一种具有高复发性的肿瘤样疾病。在这种情况下，基于成像的子宫内膜异位症准确诊断可通过改进手术方案帮助临床医生根除该病。然而，尽管造影剂可提高磁共振成像（MRI）中感兴趣组织的可视性，但子宫内膜异位症中生物标志物的缺乏阻碍了用于其靶向成像和诊断的造影剂的开发。在此，针对子宫内膜异位症中富集的血管内皮生长因子（VEGF），我们开发了一种用贝伐单抗修饰的靶向MRI造影剂，即NaGdF@PEG@贝伐单抗 - Cy5.5纳米颗粒（NPBCNs），用于检测子宫内膜异位症。NPBCNs在子宫内膜细胞中显示出可忽略不计的细胞毒性以及对VEGF的高亲和力。此外，注射后3天，NPBCNs在大鼠的子宫内膜异位症病变的T2加权图像MRI中产生了强烈的信号增强，这在同一天的组织病理学染色结果和荧光成像中得到了证实。我们的方法能够使NPBCNs有效地靶向子宫内膜异位症，从而避免漏诊。
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