慢性髓性白血病中的 CD4 T 细胞表现出 MHC Ⅱ类依赖性和 IFN-γ依赖性细胞毒性。
CD4 T cells in chronic myeloid leukemia present MHC class II-dependent and IFN-γ-dependent cytotoxic capacity.
机构信息
Department of Hematology, Affiliated Dongguan People's Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, 523059, P.R. China.
Department of Hematology, Institute of Hematology, School of Medicine, The First Affiliated Hospital of Xiamen University, Medical College of Xiamen University, Xiamen, Fujian, 361003, P.R. China.
出版信息
Med Oncol. 2023 Jul 27;40(9):253. doi: 10.1007/s12032-023-02123-5.
At present, many therapeutic schemes have been used to improve the prognosis of patients with chronic myeloid leukemia (CML), but response remains poor in a small group of patients. CD4 T cell-mediated cytotoxicity has been found in various autoimmune diseases. This study analyzed the characteristics of CD4 T cell mediated cytotoxicity in CML patients and healthy people. The cytotoxicity of CD4 T cells was tested in using two CML cell lines, including the MHC class II-deficient K562 cells and the MHC class II-expressing KU812 cells. CD4 T cell-mediated lysis was minimal in K562 cells but was much higher in KU812 cells. In CML patients, the level of CD4 T cell-mediated lysis was limited to a certain level. Interestingly, pre-treating KU812 cells with IFN-γ could significantly elevate the expression of MHC class II and elevate the level of CD4 T cell-mediated lysis. Overall, these data indicated CD4 T cells could become a potential candidate for cytotoxic elimination of CML cells.
目前，已经有许多治疗方案被用于改善慢性髓性白血病（CML）患者的预后，但仍有一小部分患者的反应较差。在各种自身免疫性疾病中都发现了 CD4 T 细胞介导的细胞毒性。本研究分析了 CML 患者和健康人 CD4 T 细胞介导的细胞毒性的特征。使用两种 CML 细胞系（包括 MHC II 缺陷的 K562 细胞和 MHC II 表达的 KU812 细胞）测试了 CD4 T 细胞的细胞毒性。在 K562 细胞中，CD4 T 细胞介导的裂解作用最小，但在 KU812 细胞中则高得多。在 CML 患者中，CD4 T 细胞介导的裂解作用限制在一定水平。有趣的是，用 IFN-γ 预处理 KU812 细胞可显著提高 MHC II 的表达，并提高 CD4 T 细胞介导的裂解作用水平。总的来说，这些数据表明 CD4 T 细胞可能成为 CML 细胞细胞毒性消除的潜在候选者。
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